组织样品处理方法对肌肉腺苷酸、乳酸和丙酮酸测定值的影响  

Signifioant Influence of Sample Processing on the Assay of Adenine Nucleotides, Lactate and Pyruvate in Rat Muscle

作　　者：叶冀明 郭亚东[1] 朱月春[1] 吴双凤[1] 许贤芳[1] 

机构地区：[1]昆明医学院实验中心分析生物化学室

出　　处：《昆明医学院学报》1992年第1期16-19,共4页Journal of Kunming Medical College

摘　　要：本文探讨了样品处理方法对大鼠肌肉组织腺苷酸、乳酸和丙酮酸测定值的影响。结果表明:肌肉取出后迅速在液氮中夹冻并研磨成粉,在4℃用玻璃匀浆器匀浆能较完全地破碎细胞膜,得到满意的测定值。再经超声波处理,线粒体等亚细胞结构完全破碎,ATP和总腺苷酸(TAN)的测定值增加20％,乳酸和丙酮酸变化不大。超声波处理在1min以内AMP和ADP变化不明显,时间过长则随时间而升高。An ideal assay of adenine nucleotides, lactate and pyruvate in the extract of rat freeze-clamped muscle was achieved. Electromicroscopy showed that the cell membrane was destroyed by powdering the muscle in liquid N_2 and then homogenizing for 1 min. An additien of ultrasonic treatment for 1 min could thoroughly break the subcellular structures and lead to a remarkable increase in ATP and total adenine nucleotides with a little change of lactate and pyruvate. The prolongation of the treatment for two more minutes, however, could result in the breakdown of ATP.

关 键 词：样品处理 腺苷酸 乳酸 丙酮酸 肌肉 

分 类 号：Q593[生物学—生物化学]