邻苯二甲醛的荧光光度法测定  

Fluorimetric Determination of o-Phthalaldehyde

作　　者：郑洪[1] 朱广华[1] 

机构地区：[1]东南大学公共卫生学院,南京市丁家桥路87号210009

出　　处：《光谱实验室》2003年第4期533-536,共4页Chinese Journal of Spectroscopy Laboratory

摘　　要：在 p H11.0的缓冲介质中 ,邻苯二甲醛与 l-组氨酸形成的复合物在 5 4 0 nm处发射出强烈荧光 ,由此建立了邻苯二甲醛的荧光光度分析新方法 ,并系统的研究了邻苯二甲醛与 l-组氨酸结合的反应条件。本法检出限为 1.2× 10 -8mol/ L,荧光强度与浓度在 4 .0× 10 -8— 4 .0× 10 -6 mol/ L范围内呈良好的线性关系。将该法用于消毒剂中邻苯二甲醛的测定 ,回收率为 95 .8%— 10 6.5 %。A new fluorimetric method for the determination of o-phthalaldehyde was established based on the fluorescent reaction of o-phthalaldehyde with l-histidine in pH 11.0 buffer solution under optimized conditions.The linear relationship between the fluorescence intensity and the concenntration of o-phthalaldehyde was in the range of 4.0×10 -8—4.0×10 -6mol/L.The detection limit was 1.2×10 -8mol/L.The new method was satisfactorily used in the determination of o-phthalaldehyde in disinfectant with recoveries in the range of 95.8%—106.5%.

关 键 词：邻苯二甲醛 荧光光度法 测定 消毒剂 L-组氨酸 

分 类 号：TQ244.1[化学工程—有机化工] R187[医药卫生—流行病学]