利用组织芯片技术分析肝细胞癌组织P21^(WAF1/CIP1)、PCNA和Ki-67的表达及意义  

Expression of P21^(WAF1/CIP1),PCNA,Ki-67 in HCC by tissue chip assay

作　　者：朱争艳[1] 高英堂[1] 杜智[1] 徐东波[1] 齐之丽[1] 景丽[1] 张文[1] 

机构地区：[1]天津市第三中心医院,天津300170

出　　处：《临床肝胆病杂志》2003年第4期245-246,共2页Journal of Clinical Hepatology

摘　　要：利用组织芯片技术检测P2 1WAF1/CIP1、PCNA与Ki - 6 7在肝细胞癌组织中的表达与分布 ,初步探讨P2 11WAF1/CIP1、PCNA与Ki- 6 7在肝细胞癌发生机制以及预后评估方面的作用。采用免疫组化技术结合临床病理资料检测了 6 1例肝癌和 5 0例癌旁组织的P2 1WAF1/CIP1、PCNA、Ki- 6 7的表达情况。PCNA和Ki- 6 7表达与肝癌的分化程度相关 ,PCNA与肝癌的侵袭转移潜力有关 (P <0 0 5 ) ,P2 1WAF1/CIP1在实验中未见有明显差异 ,但在表达程度与肝癌组织的分化程度之间亦存在着一定的相关关系。应用组织芯片高效检测临床组织样本具有快速、方便、经济、准确的优点。P2 1WAF1/CIP1、PCNA与Ki- 6To detect the expressions and significance of P21 WAF1/CIP1, PCNA, Ki-67 in HCC by tissue microarray assay. Immunohistochemic stain combined with histological pathology were used to observe the P21 WAF1/CIP1, PCNA and Ki-67 expressions in 61 cases of HCC and 50 cases of peritumorous tissues. The expressions of PCNA, Ki-67 were correlated with histological differentiation, whereas, the expressions of PCNA had relation with potential of invasion and metastasis of HCC. Expressions of P21 WAF1/CIP1, PCNA and Ki-67 may be related to the development of hepatocellular carcinoma and it is feasible to utilize tissue microarray for rapid, economic and accurate screening of clinical tissue specimens on a large scale.

关 键 词：组织芯片技术 分析 肝细胞癌组织 P21^WAF1/CIPI PCNA KI-67 表达 

分 类 号：R735.7[医药卫生—肿瘤]