Expression and localization of VCX/Y proteins and their possible involvement in regulation of ribosome assembly during spermatogenesis  

作　　者：SHENGWEIZOU JIANCHAOZHANG XIAODONGZHANG SHIYINGMIAO SHUDONGZONG QISHENG LINFANGWANG 

机构地区：[1]NationalLaboratoryofMedicalMolecularBiology,InstituteofBasicMedicalSciences,ChineseAcademyofMedicalSciences,PekingUnionMedicalCollege,5DongDan3-tiao,Beijing100005,China [2]NationalResearchInstituteforFamilyPlaning,WHOCollaborationCenterforResearchinHumanRe-production,12DaHuiSi,Beijing100081,China

出　　处：《Cell Research》2003年第3期171-177,共7页细胞研究（英文版）

基　　金：supported by grants from the Special Fund for Major State Basic Research Project(No.G1999055901);National Natural Sciences Foundation of China(No.30240019);National High Technology Research and Technology of China(No.2001AA221131);State Ministry of Science and Technology Program(No.2002BA711A011.

摘　　要：Variable Charge X/Y (VCX/Y) is a human testis-specific gene family that localized on X and Y chromosomes. In this study, VCY protein was expressed in E. coli in the form of glutathione-S-transferase (GST) fusion protein. With the purified fusion protein as antigen, the anti-GST-VCY antibody was generated and the localization of VCY protein in human testis was determined by immunohistochemistry. In the testis seminiferous epithelium. VCY proteins were highly expressed in nuclei of germ cells. Using propidium iodide staining and green fluorescent protein (GFP) tag technologies, VCY and VCX-8r proteins were mainly localized in the uucleoli of COST cells. In addition, the colocalization for VCY and VCX-8r in COS7 cells was also observed. With VCY cDNA as bait, a cDNA fragment of acidic ribosomal protein PO was obtained using yeast two-hybrid system. All the information above indicates that VCX/Y protein family might be involved in the regulation of ribosome assembly (hiring spermatogenesis.

关 键 词：VCX/Y protein SPERMATOGENESIS nucleoli colocnlization. 

分 类 号：Q132.7[生物学—普通生物学] Q492.4