哺乳动物细胞表达系统及其研究进展  

Mammalian expression systems and its progresses

作　　者：刘定燮[1] 周晓巍[1] 黄培堂[1] 

机构地区：[1]军事医学科学院生物工程研究所,北京100071

出　　处：《生物技术通讯》2003年第4期308-311,共4页Letters in Biotechnology

基　　金：中国博士后科研基金资助

摘　　要：从表达载体、宿主细胞、表达系统的类型及选择等四个方面对哺乳动物细胞系统进行了综述。对相关的研究进展,如高效启动子的发现和改构、新型细胞株的建立及诱导表达调控系统亦作了简要介绍。通过这些阐述,期望能建立一个简洁而清晰的有关该表达系统的框架,对在实验中决定选取何种载体、细胞株或何种表达方式时能够有所提示。Mammalian cell expression system has been essential to the production of recombinant proteins as well as tools in research on protein structure and function.We here make a mini review on this expression system from four aspects,including expression vector,cell host,type and choice of expression system.Some related progresses are al-so discussed here,such as induced expression system,newly developed strong promoters and engineered cell lines for high protein production.By this elucidation,we hope to be able to make a simple and clear profile on the ex-pression system,which maybe helpful in deciding to select which type of promoter,vector,cell host and expression mode for a gene expression experiment.

关 键 词：哺乳动物细胞 表达系统 宿主细胞 表达载体 

分 类 号：Q78[生物学—分子生物学]