志贺毒素保护性抗原的基因克隆与序列分析  

Cloning and structural analysis of the genes of Shiga toxin protective antigens

作　　者：邢洪光[1] 荫俊[1] 王慧[1] 侯晓军[1] 宋伟[1] 

机构地区：[1]军事医学科学院微生物流行病研究所,北京100071

出　　处：《生物技术通讯》2003年第4期285-287,共3页Letters in Biotechnology

摘　　要：根据GenBank报道的志贺毒素A、B亚单位基因序列设计合成两对引物,以Ⅰ型痢疾志贺菌的基因组为模板经PCR扩增获得3条DNA序列,将其分别插入pMD18-T载体,测序证明所获得的3条序列为志贺毒素A、B亚单位基因及其串联片段,与GenBank中相应序列比较,同源性分别为99.5%、100%和99.8%。通过计算机软件对所推测的氨基酸序列进行了蛋白质参数分析。志贺毒素保护性抗原基因的获得为志贺毒素的免疫学研究准备了必要的材料。Two pairs of primer were designed and synthesized according to the genes of Shiga toxin subunit A and subunit B in GenBank.Three sequences were amplified from the genome of Shigella dysenteriae typeⅠby PCR and inserted into pMD18-T Vector.Sequencing proved the three sequences are genes of subunit A and subunit B of Shiga toxin and their tandem.The identities of the three sequences to their corresponding sequences in GenBank are99.5%,100%and99.8%respectively.The analyses on parameters for proteins of the sequences obtained were per-formed.The cloned genes of Shiga toxin protective antigens provided essential material for the immunological re-search of Shiga toxin.

关 键 词：志贺毒素 保护性抗原 基因克隆 序列分析 表达 A亚单位 B亚单位 

分 类 号：R378.25[医药卫生—病原生物学] Q785[医药卫生—基础医学]