牙本质涎磷蛋白在小鼠牙胚表达的原位杂交研究  

Expression of dentin sialophosphoprotein during mouse teeth development studied by in situ hybridization

作　　者：张蓉[1] 肖明振[1] 赵守亮[1] 徐平西[2] 张春宝[1] 汪平[1] 

机构地区：[1]西安第四军医大学口腔医学院,710032 [2]西安第四军医大学口腔医学院神经生物教研室,710032

出　　处：《实用口腔医学杂志》2003年第4期346-348,共3页Journal of Practical Stomatology

基　　金：国家自然科学基金 (3980 0 1 55);国家教育部回国人员基金 (2 0 0 0HG0 0 3)

摘　　要：目的 :探讨牙本质涎磷蛋白 (dentinsialophosphoprotein ,DSPP)mRNA在牙齿发育过程中的作用。方法 :采用原位杂交技术 ,观测小鼠牙齿发育各阶段标本中DSPPmRNA的表达。结果 :DSPP在成牙本质细胞的表达始于钟状中期 ,并贯穿以后的各个时期 ;此阶段 ,它还在前成釉细胞及成釉细胞中具有一过性表达。结论 :DSPP在牙胚发育过程中的表达具有时空特异性 ;Objective: To evaluate the function of dentin sialophosphoprotein (DSPP) during mouse teeth development. Methods:Expression of DSPP mRNA in the first molar of Balb/c mice at different developmental stages was detected by in situ hybridization. Resullts: DSPP mRNA in odontoblasts was detected in the samples at middle bell stage and ran through the later stages. In addition, it was detected in ameloblasts as well as in preameloblasts. Conclusion: DSPP expresses with temporospatial characters. It may play an important role in tooth morphogenesis

关 键 词：牙本质涎磷蛋白 小鼠 牙胚表达 原位杂交 牙齿发育 牙本质磷蛋白 牙本质涎蛋白 

分 类 号：R780.2[医药卫生—口腔医学]