釉基质蛋白对成骨细胞碱性磷酸酶和I型胶原合成的影响  

The effects of enamel matrix proteins on the synthesis of alkaline phosphatase and type I collagen in osteoblasts

作　　者：束蓉[1] 邬春兰[2] 

机构地区：[1]上海第二医科大学附属第九人民医院口腔内科,200011 [2]上海第二医科大学附属宝钢医院口腔科

出　　处：《实用口腔医学杂志》2003年第4期366-368,共3页Journal of Practical Stomatology

摘　　要：目的 :观察釉基质蛋白 (EMPs)对成骨细胞碱性磷酸酶和I型胶原合成的影响 ,进一步探讨EMPs促进牙槽骨再生的机制。方法 :体外培养MC3T3 E1成骨细胞 ,在培养液中加入不同浓度的EMPs ,用酶动力学方法和MTT法作碱性磷酸酶 (ALP)比活性测定 ,同位素掺入结合细菌胶原酶消化法测定I型胶原的合成。结果 :EMPs能明显促进MC3T3 E1成骨细胞的碱性磷酸酶合成以及I型胶原合成。结论 :EMPs对成骨细胞的生物学活性有明显促进作用 。Objective:To study the effects of enamel matrix proteins (EMPs) on the biological characters of osteoblasts.Methods:MC3T3-E1 osteoblasts were cultured in vitro and exposed to EMPs at 300,400 and 500 μg/ml.Enzyme kinetic method was used to assay alkaline phosphatase (ALP) synthesis and 3H-proline labelling combining collagenase digestion method was used to assay type I collagen synthesis.Results:The results manifested that ALP level in EMPs treated groups was higher than that in control group (P<0.01) .The synthesis of type I collagen in EMPs treated groups was signifiantly increased (P<0.01). Conclusions: EMPs may stimulate the function of osteoblasts and may play an important role in the regeneration of alveolar bone.

关 键 词：釉基质蛋白 成骨细胞碱性磷酸酶 I型胶原 合成 牙槽骨再生 牙周组织再生 

分 类 号：R780.2[医药卫生—口腔医学]