利用特异性单克隆抗体测定血清大分子硷性磷酸酶同工酶对肝癌诊断价值的研究  

A study of the value for the measurement of serum high molecular weight alkaline phosphatase using a specific monoclonal antibody in patients with carcinoma of the liver.

作　　者：潘其兴[1] 邓景惕[2] 康鲁东[2] 刘清明[2] 张震 

机构地区：[1]附属医院内科 [2]山东医科大学生化教研室

出　　处：《临床肝胆病杂志》1992年第1期8-10,共3页Journal of Clinical Hepatology

摘　　要：利用特异性单克隆抗体,采用免疫催化方法,对70例肝癌(原发性肝癌49例,转移性肝癌21例)病人,进行血清大分子硷性磷酸酶(HMAP)同工酶活力与甲胎蛋白(AFP)定量的测定,且与24例胆囊炎、19例急性肝炎及104例正常人的检测做对比分析。结果显示:70例肝癌病人的血清HMAP阳性率为96%,其中原发性肝癌的阳性率96%,转移性肝癌的阳性率95%。与AFP定量测定比较,HMAP诊断肝癌,尤其转移性肝癌,具有更高的敏感性及特异性。A new immunocatalytical method using a specific monoclonal an- tibody has been developed to detect high molecular weight alkaline phosphatase (HMAP) isoenzyme in the sera of 70 patients with carci- noma of the liver. Of them, 49 patients suffered from primary carci- noma of the liver and 21 patients metastatic carcinoma of the liver. In the meantime, the serum AFP levels were determined in 49 of the 70 patients with carcinoma of the liver. For control analysis, a large number of sera in 24 patients with cholecystitis, 19 patients with acute heptitis and 104 normal people were assayed to evaluate the significan- ce of serum HMAP in relation to total alkaline phosphatase activities and to the differential diagnosis. The results showed that the positive rate of the serum HMAP was 96% in all of the patients with carcino- ma of the liver, 96% in 49 patients with primary carcinoma of the liver and 95% in 21 patients with metastatic carcinoma of the liver. Compared with the detection rate using the measurement of serum AFP levels, we found that the serum HMAP measurement had the added advantages of increased sensitivity and specificity.

关 键 词：肝肿瘤 癌 诊断 HMAP 

分 类 号：R735.04[医药卫生—肿瘤]