聚乙烯醇-海藻酸盐共固定化冰核活性细菌——黄单胞菌TS206的研究  

Study on PVA-alginate Co-immobilization of Xanthomonas ampelina TS206

作　　者：陈庆森[1] 刘健[1] 

机构地区：[1]天津商学院生物工程系,天津300134

出　　处：《微生物学报》2003年第4期492-497,共6页Acta Microbiologica Sinica

基　　金：国家自然科学基金资助项目 ( 3 9770 5 80 ) ;天津市自然科学基金资助项目 ( 0 2 3 80 3 3 1 1 )~~

摘　　要：冰核活性细菌固定化在食品冷冻浓缩中的应用具有重要意义 ,冰核活性和抗渗漏能力是衡量其性能的两个重要技术指标。研究采用PVA和海藻酸盐作为固定化载体 ,通过两者优良性能的互补而建立对冰核活性细菌XanthomonasampelinaTS2 0 6的共固定化技术。结果表明 ,细胞投入量对冰核活性有较大影响 ,基础固定化条件对固定化技术指标的综合评分影响程度大小的顺序依次为 :海藻酸钠浓度 >硼酸浓度 >PVA浓度 >CaCl2 浓度 ,各因素的较优水平是 :海藻酸钠浓度 1 % ,硼酸浓度 5 % ,PVA浓度 8% ,CaCl2 浓度 1 1 % ;研究还发现冰核活性与固定化凝胶珠的添加量正相关 ,与固化时间相关性较小 ,渗漏量受固定化凝胶珠的添加量和固化时间影响不显著。Ice nucleation activity and anti-leaking capability are both important technical parameters in INA (Ice nucleation active bactetia)bacteria immobilization which can be adopted on freezing concentrate. Both PVA and alginate are good medium for immobilization. They can be used on co-immobilization of ice nucleation-active bacteria ( Xanthomonas ampelina TS206). The results showed that quantity of embedding affects ice nucleation activity greatly. The order of importance to comprehensive scores of technical standard should be concentration of Sodium alginate>Boric acid>PVA>CaCl_2. The optimized concentration are PVA 8%,sodium alginate 1%,CaCl_2 1.1% and boric acid 5%. A conclusion can be draw that ice nucleation activity increases with the number of beads and shows little pertinency with the time of immobilization, whereas anti-leaking capability can be influenced faintly by the number of beads and the time of immobilization.

关 键 词：聚乙烯醇 海藻酸盐 固定化 冰核活性 黄单胞菌 

分 类 号：TS201[轻工技术与工程—食品科学]