钙磷脂结合蛋白Ⅱ在人食管鳞状细胞癌中的表达  

Expression of annexin Ⅱ in human esophageal squamous cell carcinoma

作　　者：张洵[1] 支会英[2] 张健[2] 王秀琴[2] 周传农[2] 吴旻[2] 孙耘田[1] 刘芝华[2] 

机构地区：[1]中国医学科学院中国协和医科大学肿瘤医院肿瘤研究所病理科,北京100021 [2]中国医学科学院中国协和医科大学肿瘤医院肿瘤研究所细胞生物室分子肿瘤学国家重点实验室,北京100021

出　　处：《中华肿瘤杂志》2003年第4期353-355,共3页Chinese Journal of Oncology

摘　　要：目的　探讨钙磷脂结合蛋白Ⅱ (annexinⅡ )在人食管鳞状细胞癌中的表达变化及其与食管鳞状细胞癌临床病理的相互关系。方法　应用半定量RT PCR、免疫组织化学和光密度扫描等方法 ,从mRNA和蛋白两个水平检测食管鳞状细胞癌中annexinⅡ的表达 ,并分析其与肿瘤分化程度的关系。结果　annexinⅡ在mRNA和蛋白水平上均在食管鳞癌组织中低表达 (P <0 .0 1,P <0 .0 5 ) ,在中分化和低分化肿瘤中 ,蛋白表达明显低于高分化肿瘤 (P <0 .0 5 )。结论　annexinⅡ在食管鳞状细胞癌中低表达 ,可能与鳞状细胞的分化程度有关。Objective To evaluate the expression of annexin Ⅱ in human esophageal squamous cell carcinoma (ESCC) and its relation with clinicopathological data. Methods The expression of annexinⅡ mRNA and protein in paired cancer tissues and their adjacent quasi normal tissues were detected by RT PCR, immunohistochemical method and densitometric scanning. The relation between annexinⅡ expression and the status of tumor differentiation was analyzed. Results The expression of annexinⅡ was significantly lower in the tumor tissue than that in its paired normal counterpart both in mRNA and protein level ( P <0.05, P <0.01). The protein expression of annexinⅡ was significantly lower in moderately and poorly differentiated tumors than those in well differentiated ones ( P <0.05). Conclusion Down regulation of annexinⅡ in esophageal carcinogenesis may play an important role in squamous cell differentiation. [

关 键 词：食管癌 鳞状细胞癌 钙磷脂结合蛋白Ⅱ 肿瘤分化程度 

分 类 号：R735.1[医药卫生—肿瘤]