双歧杆菌对实验性大肠癌NF-κB和IκBα的影响  

Influence of bifidobacterium on NF-κB and I κBα in experimental large bowel carcinoma

作　　者：王立生 朱惠明 潘令嘉[2] 黄勋 罗伟香 马晓东[3] 张亚历[2] 周殿元[2] 

机构地区：[1]暨南大学医学院第二附属医院深圳市人民医院消化内科,广东深圳518020 [2]第一军医大学南方医院全军消化内科研究所,广东广州510515 [3]第一军医大学基础部中心实验室,广东广州510515

出　　处：《中国病理生理杂志》2003年第8期1062-1064,共3页Chinese Journal of Pathophysiology

基　　金：广东省自然科学基金资助项目 (No .994 0 6 6 )

摘　　要：目的 :阐明青春型双歧杆菌的体内抑瘤机理。方法 :以大肠癌裸鼠移植瘤为动物模型 ,用激光共聚焦显微镜和免疫组化检测了大肠癌组织NF -κB及其抑制性蛋白IκBα的活性。结果 :双歧杆菌注射组大肠癌移植瘤NF -κB的阳性细胞密度明显低于肿瘤对照组 (P <0 0 1) ;而IκBα的表达则相反 ,双歧杆菌注射组大肠癌IκBα的平均荧光强度显著高于肿瘤对照组 (P <0 0 1)。结论 :青春型双歧杆菌体内能抑制大肠癌IκBα的降解 ,进而阻止NF -κB的活化。AIM: To explore the antitumor mechanisms of bifidobacteria adolescence in vivo. METHODS: The activity of NF-κB and its inhibiting protein I κBα of large bowel carcinoma tissues was detected by using laser scanning confocal microscope and immunohistochemistry. RESULTS: The positive cell density of NF-κB of large bowel carcinoma transplantation tumors in bifidobacterium injection group was markedly lower than that in tumor control group( P< 0.01).The expression of I κBα was contary in the two group. The average fluorescent strength of I κBα of large bowel carcinoma in bifidobacterium injection group was significantly higher than that in tumor control group( P< 0.01). CONCLUSION: Bifidobacteria adolescence could inhibit the degrade of I κBα and the activition of NF-κB in large bowel carcinoma in vivo.

关 键 词：二裂菌属 肠肿瘤 NF—κB 

分 类 号：R363[医药卫生—病理学]