肿瘤坏死因子-α在中耳胆脂瘤组织中的表达  

Study of TNF-α Destructive Action to the Bone in Patient with Cholesteatoma in Middle Ear

作　　者：李华清[1] 杨清绪[1] 廖艳萍[1] 麦平青[1] 朱全胜[2] 

机构地区：[1]广东省惠州市中心医院,惠州市516001 [2]中山医科大学病理教研室

出　　处：《医药论坛杂志》2003年第14期9-10,共2页Journal of Medical Forum

摘　　要：目的　研究肿瘤坏死因子 -α(TNF α)在中耳胆脂瘤组织中的表达 ,探讨其在中耳胆脂瘤骨质破坏中的作用。 方法　 采用免疫组织化学SABC染色法和计算机图像定量分析法 ,对 2 0例中耳胆脂瘤和 10例正常外耳道皮肤中TNF -α的表达情况进行观察。 结果　TNF α不仅在胆脂瘤上皮的各层细胞胞浆中 ,而且在皮下组织的炎性细胞中都有高度表达。定量分析显示胆脂瘤上皮中TNF α的含量显著高于外耳道正常皮肤 (P <0 .0 5 )。 结论　TNF α高表达可能提示胆脂瘤引起骨质破坏的一个因素。Objective To study the expression of TNF-α in the cholesteatoma and elucidate the role of TNF-α in the destruction of bone. Methods All samples from 20 cholesteatoma cases and 10 normal cases were examined by immunohistochemical SABC method and analysed by computer image.Results In 20 cholesteatoma cases,TNF-α expressed in the cyloplasm of the full epithelial tissue cells,subcutaneous inflammatory cells and fibroblast. The results of the computer image quantitative analysis system showed that the mean optical density of TNF-α was 0.3427±0.0686 in the cholesteatoma epithelial tissue and 0.1940±0.0563 in normal skin epithelial tissue respectively,both of which had significantly differentiation(P>0.05).Conclusion The high expression of TNF-α may be a factor of the destruction of bone in patient with cholesteatoma.

关 键 词：肿瘤坏死因子-Α 中耳胆脂瘤 表达 骨质破坏 

分 类 号：R739.61[医药卫生—肿瘤]