水稻Rubisco及其活化酶的免疫金标定位  

Immunogold localization of Rubisco and Rubisco activase in leaves of rice

作　　者：王妮妍[1] 蒋德安[1] 张峰[1] 洪健[2] 费万辛[1] 

机构地区：[1]浙江大学生物系,浙江杭州310029 [2]浙江大学生物技术研究所,浙江杭州310029

出　　处：《浙江大学学报（农业与生命科学版）》2003年第4期387-391,共5页Journal of Zhejiang University:Agriculture and Life Sciences

基　　金：国家自然科学基金资助项目(39970440).

摘　　要：采用免疫胶体金标记电镜技术对水稻叶片中的Rubisco及其活化酶进行细胞器定位,结果表明Rubisco主要分布于叶绿体,Rubisco活化酶特异性分布于叶绿体和线粒体中,预示Rubisco活化酶可能具有除活化钝化态Rubisco以外的其他功能.The enzymes of Rubisco and Rubisco activase were localized using immunogoldlabeled method and electron microscope techniques. The results showed that Rubisco was mainly located in choloroplast, Rubisco activase, however, specially labeled both in chloroplast and mitochondria, which implied that Rubisco activase has other functions besides activating inactive Rubisco in photosynthesis.

关 键 词：水稻 RUBISCO RUBISCO活化酶 免疫胶体金标记电镜技术 细胞器 定位 叶绿体 线粒体 光合作用 

分 类 号：Q949.714.2[生物学—植物学] Q945.11