血清雌二醇放射免疫试剂盒的研制  

RADIOIMMUNOASSAY FOR ESTRADIOL IN SERUM

作　　者：张丁联[1] 潘家荣[1] 王斌[1] 

机构地区：[1]中国农业科学院原子能利用研究所,农业部农业核技术与航天育种重点开放实验室,北京100094

出　　处：《核农学报》2003年第4期304-307,共4页Journal of Nuclear Agricultural Sciences

摘　　要：制备了雌二醇抗体 (抗E2 6 CMO BSA)和12 5I放射配体E2 6 CMO histamine 12 5I,建立了血清雌二醇放射免疫分析方法 ,其特点是通过微量标记法提高标记物比活度 ,不用乙醚萃取直接测定血清样本 ,简化了测量步骤 ,缩短了测量时间 ,且试剂稳定时间可延长至 2个月以上。应用本法可以快速测量动物及人的血清样本 ,2 5～ 3h即可得出结果。A sensitive radioimmunoassay for estradiol was set up without extraction The antisera were rabbits anti estradiol 6 CMO BSA with a cross reaction ratio of 0 6% against estriol Pre synthesized estradiol 6 CMO His was labeled with 125 I in a tiny volume (17 5μl) Highly purified radioactive (3550μCi/μg) estradiol 6 CMO His 125 I was obtained This estradiol radioimmunoassay kit is stable for at least two months It can be used for studying animal endocrinology and clinical endocrinology

关 键 词：血清 雌二醇 抗体 放射免疫试剂盒 研制 微量标记法 天然雌激素 食品 监测 

分 类 号：S852.2[农业科学—基础兽医学]