促红细胞生成素增加K562细胞转铁蛋白受体的表达及其对细胞周期的影响  

Erythropoitin increases transferrin receptor expression and the impact of erythropoitin on K562 leukemic cell cycle

作　　者：周密[1] 廖清奎[1] 李丰益[1] 高举[1] 符仁义[1] 罗春华[1] 李强[1] 贾苍松[1] 

机构地区：[1]四川大学华西第二医院儿科,成都610041

出　　处：《中华儿科杂志》2003年第7期528-530,共3页Chinese Journal of Pediatrics

摘　　要：目的　探讨重组人促红细胞生成素 (recombinanthumanerythroipoitin ,rhEPO)和铁对白血病细胞K5 6 2转铁蛋白受体 (transferrinreceptor,TfR)即CD71 抗原表达的影响 ,及其相同处理因素对K5 6 2细胞周期变化的影响。方法　体外培养K5 6 2细胞并加rhEPO等处理因素 ,分别在 2 4h和 72h终止处理 ,各处理组细胞分别与FITC荧光标记的CD71 单抗孵育 ,或与DNA染料碘化丙啶作用。通过流式细胞分析技术检测CD71 抗原的表达和细胞周期 ,观察各组TfR表达情况和S期细胞百分率。结果　 (1)不同浓度的rhEPO培养K5 6 2细胞 72h ,均可使TfR表达增加 ,与同期空白对照组相比差异有显著意义 (P <0 0 5 )。 (2 )单独使用rhEPO(5U ml)或rhEPO(5U ml) +FeCl3(10 0 μmol L)培养细胞 72h可使S期细胞百分率上升 ,且与空白对照组相比差异有显著意义 (P <0 0 5 )。Objective Functionally, erythropoietin (EPO) can promote the proliferation and growth of erythroid progenitor cells, and it is widely used in the treatment of anemia in chronic diseases caused by tumor and inflammation. However, it is unclear whether EPO has any effect on tumor cell iron metabolism and tumor cell proliferation. The purpose of this study was to explore the effects of recombinant human EPO (rhEPO) on the expression of transferrin receptor (TfR, CD 71 antigen) of leukemic cell K562 and its relation to cell cycle. Methods In vitro culture of K562 cell was performed with additions of various concentrations of rhEPO and Fe. Treatments were terminated at 24 h and 72 h, respectively. Then each group of cells was incubated with FITC-IgG antibody to CD 71 or PI, a kind of DNA dye. And TfR expression and DNA synthesis status were analyzed by flow-cytometry. Results (1)The expression of TfR by K562 cells increased significantly when incubated for 72 h with different concentrations of rhEPO. The measurement values of 5 U/ml, 10 U/ml and 20 U/ml groups were 12.2±1.40, 10.7±0.99 and 11.1±0.90, respectively. They were markedly increased when compared with that of control group (6.27±0.11, P<0.05). (2)When incubated with rhEPO (5 u/ml) alone or combined with FeCl 3 (100 μmol/L), the percentages of cells in S phase were 51.1% and 59.6%, respectively. They significantly increased when compared with that of control group (42.9%, P<0.05). Conclusions Iron is very important for the proliferation of both normal cells and leukemic cells. It is essential to the activity of ribonucleotide reductase (RR). The authors hypothesized that rhEPO would increase the expression of TfR and intracellular iron content of leukemic cells, which would enhance the DNA synthesis and cell proliferation. Therefore, the clinical application of rhEPO to promote erythropoiesis of cancer patients should be cautious.

关 键 词：促红细胞生成素 K562细胞 转铁蛋白受体 表达 细胞周期 影响 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]