联苯胺对大鼠肝脏线粒体同工酶的影响  

The Effect of Benzidine on Mitochondrial Isozymes of Liver of Mice

作　　者：戴捷[1] 梅启明[2] 吴峰 杨芰[2] 马辉文[2] 

机构地区：[1]湖北农学院,湖北荆州434025 [2]武汉大学药学院,湖北武汉430072 [3]武汉市医学会<中国医院药学杂志>编辑部,湖北武汉430014

出　　处：《药物生物技术》2003年第4期226-228,共3页Pharmaceutical Biotechnology

摘　　要：采用不同浓度联苯胺 (5 0mg kg ,10 0mg kg ,2 0 0mg kg)对大鼠进行腹腔注射 ,研究了大鼠肝脏线粒体Ca2 + ATP酶和超氧化物歧化酶 (SOD)活性及其同工酶的变化。结果表明在联苯胺作用下这 2种酶的活性表现为低浓度被激活 ,高浓度被抑制 ;在同工酶谱中出现了ATP1、SOD3和SOD4这 3种酶的特异性表达。分析认为 ,联苯胺对大鼠肝脏线粒体的毒性作用可能为一种混合机制。The effect of different concentration benzidine (50mg/kg,100mg/kg,200mg/kg) to Ca 2+-ATPase, superoxide dismutase(SOD) and their isozymes of liver of mice are discussed through belly injection. The result indicates that the activities of the two enzymes are all activated by low concentration benzidine and restrained by high concentration benzidine, on the other hand three symbol enzymes ATP1?SOD3 and SOD4 are found in the pattarn of the two isozymes. This concludes that the effect of benzidine to mitochondria maybe belong to mix mechanism.

关 键 词：联苯胺 线粒体 CA^2+-ATP酶 SOD酶 同工酶 大鼠 

分 类 号：R965[医药卫生—药理学]