肝外胆管癌组织BAG-1与BAD表达与凋亡调控的原位定量研究  

In situ quantitative study of BAG-1 and BAD in extrahepatic cholangiocarcinoma

作　　者：闫庆国[1] 师建国[1] 黄高昇[1] 张传山[1] 李青[1] 胡沛臻[1] 王文亮[1] 

机构地区：[1]中国人民解放军第四军医大学西京医院病理科,陕西省西安市710033

出　　处：《世界华人消化杂志》2003年第8期1144-1147,共4页World Chinese Journal of Digestology

基　　金：国家自然科学基金资助课题;No.39270744

摘　　要：目的:检测凋亡相关蛋白BAG-1与BAD在肝外胆管癌中的表达,探讨BAG-1与BAD在肝外胆管癌细胞凋亡调控中的作用以及与胆管癌分化的关系。方法:采用免疫组织化学方法(DAKO EnVision^(TM) Systems),结合计算机图像分析系统与凋亡细胞计数,在组织原位定量检测42例肝外胆管癌的凋亡状况及BAG-1与BAD蛋白的表达水平。结果:在24例高分化、13例中分化和5例低分化的肝外胆管癌中,免疫组化定量检测显示BAG-1阳性染色的平均吸光度值分别为0.068±0.037,0.112±0.057和0.152±0.062,BAD阳性染色的平均吸光度值分别为0.079±0.053,0.138±0.058和0.165±0.061,HE染色计数典型凋亡细胞的指数分别为1.1±0.5,1.7±0.3和3.5±1.3。BAG-1、BAD的表达水平及凋亡指数在高、中、低分化的肝外胆管癌中逐级增加(P<0.05),凋亡指数与BAD的表述呈正相关(P<0.01),表明凋亡相关的BAG-1和BAD蛋白的表达与肝外胆管癌的分化程度有关,且二者可能参与了其凋亡的调控。结论:在肝外胆管癌中,凋亡相关蛋白BAG-1和BAD的表达及凋亡指数与肿瘤的分化有关。BAG-1和BAD均有可能参与其凋亡调控。AIM: To detect the expression of apoptosis related proteins BAG-1 and BAD and to investigate their functions of apoptosis modulation and relations to tumor differentation in extrahepatic cholangiocarcinoma. MFTHODS: Quantitative in situ methods of DAKO Envision^(TM) Systems immunohistochemistry in combination with computer- assisted image analysis and apoptotic cells counting were used to observe the expression levels of BAG-1 and BAD and the apoptosis indexes in 42 cases of extrahepatic cholangiocarcinoma. RESULTS: The expression levels of BAG-1 and BAD were measured with average absorbance (A) derived from the quantitative image analysis. Among 24, 13 and 5 cases of well, moderately and poorly differentiated extrahepatic cholangiocarcinomas,the average A of BAG-1 was respectively 0.068±0.037, 0.112±0.057 and 0.152±0.062, of BAD was respectively 0.079±0.053, 0.138±0.058 and 0.165±0.061, and the apoptotic indexes examined in the hematoxylin- and-eosin-stained specimens were respectively 1.1±0.5, 1.7±0.3 and 3.5±1.3. The expression levels of BAG-1 and BAD and the apoptotic indexes were increased obviously from well, moderately to poorly differentiated extrahepatic cholangiocarcinoma (P<0.05). The expression level of BAD was correlated to the apoptotic indexes (P<0.01). CONCLUSION: Expressions of BAG-1 and BAD and apoptosis indexes were all associated with differentiation of extrahepatic cholangiocarcinoma. BAG-1 and BAD may take part in the apoptosis modulation of the tumor.

关 键 词：肝外胆管癌 BAG-1 BAD 检测 凋亡相关蛋白 免疫组织化学 

分 类 号：R735.8[医药卫生—肿瘤]