大鼠急性酒精性脂肪肝造模方法的改进  

Improvement of induction method of acute alcoholic fatty liver model in rats

作　　者：冯志强[1] 沈志祥[1] 谭诗云[1] 罗和生[1] 漆楚波[2] 郭洁[1] 李海霞[1] 

机构地区：[1]武汉大学人民医院消化内科,湖北省武汉市430060 [2]湖北省肿瘤医院病理科,湖北省武汉市430070

出　　处：《世界华人消化杂志》2003年第8期1189-1192,共4页World Chinese Journal of Digestology

摘　　要：目的:为酒精性脂肪肝的研究提供有价值的模型。方法:采用高浓度酒每日灌胃,同时改进饲料配方,增加食物中脂肪含量、增加铁剂的方法。结果:造模2wk后可见肝脏轻微脂肪沉积,3wk后呈中度脂肪肝,4wk后为重度脂肪肝病变;与对照组相比,肝指数明显增大(模型组4.9±1.1;对照组4.0±0.6,P<0.05),具有统计学意义。结论:本造模方法与人类酒精性脂肪肝病变类似,方法简单易行、实验周期短,结论明确,一般实验室均可进行。AIM: To provide a valuable model for the study on acute alcoholic fatty liver. METHODS: Thirty Wistar male rats were randomized into two groups: model group (group 1,intragastric infusion of spirits,fed with high fat and iron diet),control group(group 2,intragastric infusion of saline,fed with common diet). Some rats were sacrificed after 2 and 3 weeks to evaluate the process of alcoholic fatty liver formation.All rats were sacrificed at the end of the fourth week. RESULTS: Slight fatty deposition was observed at the end of 2nd week after the experiment and moderate fatty deposition at the 3 rd week and severe fatty deposition at the 4 th week. In comparison with control group,the liver mass index was increased in the model group.There was a statistically signifiant difference between the two groups. CONCLUSION: The lesion of the acute alcoholic fatty liver in model rats is similar to that in humans.The experiment is very simple and the experiment cycle is short and the conclusion is clear.

关 键 词：大鼠 急性酒精性脂肪肝 造模方法 动物模型 实验 

分 类 号：R-332[医药卫生]