胃癌前病变演变过程中凋亡相关蛋白和PCNA的表达意义  

Expression of apoptosis-associated proteins and proliferating cell nuclear antigen (PCNA) in precancerous lesions of stomach

作　　者：伍银桥[1] 王孟薇[1] 吴本俨[1] 尤纬缔[1] 祝庆孚[2] 

机构地区：[1]中国人民解放军解放军总医院南楼消化科,北京市100853 [2]中国人民解放军解放军总医院病理科,北京市100853

出　　处：《世界华人消化杂志》2003年第8期1219-1222,共4页World Chinese Journal of Digestology

基　　金："十五"军队科研基金资助项目;No.01Z035

摘　　要：目的:探讨凋亡相关蛋白P53、Bcl-2和增生细胞核抗原(PCNA)在胃癌前病变不同转归中的作用以及在胃癌早期诊断中的意义。方法:采用免疫组化(LSAB)法检测52例胃癌患者癌前病变组织标本及56例非胃癌患者组织标本的P53、Bcl-2和PCNA的表达。结果:发生癌变者与P53、Bcl-2和PCNA阳性强度有密切关系;PCNA标记指数(PCNA LI)在癌变组表达明显高于非癌变组,差异有显著性(t=5.261,P<0.01);在癌变组,88.5％的病例有单一或多种基因表达异常,51.9％为两种或两种以上基因同时表达,与非癌变组相比有显著性差异(x^2=4.393,P<0.05)。结论:在胃癌发生过程中,P53、Bcl-2表达强度明显增加,细胞增生活性升高,且为多基因协同作用,说明在胃癌演变过程中他们起重要作用。AIM: To investigate the role of apoptosis-associated protein P53, Bcl-2, and proliferating cell nuclear antigen (PCNA) in different transformation in precancerous lesions of the stomach. METHODS: Immunohistochemical method (LSAB) was used to assess P53, Bcl-2, and PCNA expressions in specimens from patients with (cancerous group, n=52) and without (non-cancerous group, n=56) gastric cancer. RESULTS: The occurrence of gastric carcinoma had close correlation with the expression intensity of P53, Bcl-2 and PCNA, PCNA LI in cancerous group was significantly higher than that in non-cancerous group (P<0.01). In cancerous group, the majority (88.5 %) had altered expression in one or more genes; multiple altered expression was found in 51.9 %. The difference of multiple altered expression between cancerous group and non-cancerous group was statistically significant (P<0.05). CONCLUSION: Multiple altered expression of P53, Bcl-2 and PCNA and their synergistic action might play a promo- tional role in the pathogenesis of gastric carcinoma, and the cell proliferative activity was significantly increased in gastric cancer.

关 键 词：胃癌 凋亡相关蛋白 PCNA P53 BCL-2 生细胞核抗原 癌前病变 

分 类 号：R735.2[医药卫生—肿瘤] R730.3[医药卫生—临床医学]