当归注射液对脑缺血/再灌注神经元代谢物的影响  

EFFECTS OF ANGELICA SINENSIS INJECTION ON THE NEURONAL METABOLITES AND BLO OD FLOW SPEED WITHIN REPERFUSION FOLLOWING THE ISCHEMIC CEREBRAL INJURY IN RATS

作　　者：廖维靖[1] 范明[2] 杨运煌[3] 杨万同[1] 李丽云[3] 刘买利[3] 

机构地区：[1]武汉大学中南医院,湖北武汉430071 [2]军事医学科学院基础医学研究所,北京100850 [3]中国科学院武汉物理与数学研究所,湖北武汉430071

出　　处：《中国应用生理学杂志》2003年第3期209-212,共4页Chinese Journal of Applied Physiology

基　　金：国家自然科学基金项目 (39970935);湖北省科技厅攻关项目( 992P12 10 );湖北省自然科学基金资助项目(98J0 99)

摘　　要：目的 :研究当归注射液对脑缺血 /再灌注时神经元代谢物及血流速度的作用 ,阐明当归对脑缺血损伤神经修复过程的影响。方法 :雄性SD大鼠 69只 ,体重 1 50～ 1 70g ,随机分成假手术组 (n =4)、缺血损伤组 (n =30 )和当归治疗组 (n =35)。制作右大脑中动脉血供阻断 (MCAO)模型。缺血 2h后 ,当归治疗组立即腹腔注射当归注射液 (5g/kgbw)。在再灌注后 3～ 4h和 5～ 6h ,以磁共振成像 (MRI)技术研究大脑T2 加权成像 (T2 WI)和局域质子谱(1 HMRS)的变化 ,观察当归对成像和神经元代谢物N 乙酰天门冬氨酸 (NAA)、肌酸 /磷酸肌酸 (Cr/PCr)和胆碱(Cho)的影响。激光多普勒血流仪观察当归注射液对血流速度的影响 ,测定脑表面血管密度。结果 :与缺血损伤组比较 ,当归治疗组的高信号强度区信号减弱、体积小 ,NAA值大 ,Cr/NAA和Cho/NAA比值小 ,再灌注时的血流速度显著加快 ,单位面积内的血管长度增加。结论 :当归注射液加快缺血脑组织的血液循环 。Aim: To investigate the effects of Angelica sinensis injection on the ne uronal metabolites and blood flow speed within reperfusion in the ischemic cereb ral injury of rats. Methods: Sixty-nine male Sprague Dawley rat s with an average body weight of 150 to 170g were used, and were randomly divide d into three groups: sham operation group ( n =4), ischemia injury group ( n =30) underwent an operation of ischemic brain injury, Angelica-treated group ( n =35) underwent the same operation and received the treatment of Angelica s inensis injection (5 g/kg bw, i.p). The right middle cerebral artery occlusion ( MCAO) of both ischemia injury group and Angelica-treated group rats was induced by 5/0 nylon suture for 2 hours. The reperfusion was conducted for three to fou r hours and five to six hours respectively following MCAO. T 2 weighted-imagin g (T 2WI) and 1H magnetic resonance spectroscopy ( 1H MRS) were perf ormed, to study the changes in imaging and neuronal metabolites N-acetyl aspart ate (NAA), creatine/phosphocreatine (Cr/PCr) and choline (Cho) following cerebra l ischemia. The changes in blood flow speed were measured by laser Doppler flowm etry. The surface vascular density in right hemisphere were calculated. Re sults: The hyperintense signals and volume in the right cerebrum in Angelica-treated g roup decreased compared with those of the ischemia injury group, the T 2 values were decreased, and the level of NAA increased, the ratio of Cr/NAA and Cho/NAA decreased. The blood flow speed in Angelica-treated group was improved. The le ngth of brain surface vessels in group C increased. Conclusion: The Angelica sinensis injection enhanced the blood circulation in the ischemic b rain, improved the neuronal metabolisms.

关 键 词：脑缺血 磁共振成像 神经无代谢物 当归注射液 

分 类 号：R743.3[医药卫生—神经病学与精神病学] Q633[医药卫生—临床医学]