高肺血流对大鼠肺血管结构及尾加压素Ⅱ表达的影响  

IMPACT OF HIGH PULMONARY BLOOD FLOW ON PULMONARY VASCULAR STRUCTURE AND HUMAN UROTENSIN Ⅱ IN INTRAPULMONARY ARTERIES OF RATS

作　　者：齐建光[1] 杜军保[1] 李简[2] 魏冰[1] 唐朝枢[3] 

机构地区：[1]北京大学第一医院儿科,北京100034 [2]北京大学第一医院胸外科,北京100034 [3]北京大学第一医院心血管病研究所,北京100034

出　　处：《中国应用生理学杂志》2003年第3期274-277,T002,共5页Chinese Journal of Applied Physiology

基　　金：北京大学生物医学跨学科研究项目;国家重点基础研究发展规划资助项目 (G2 0 0 0 0 56 90 5)

摘　　要：目的 :探讨新型血管活性肽人类尾加压素Ⅱ (hUⅡ )在高肺血流量所致肺动脉高压大鼠肺动脉中的表达及其作用。方法 :对大鼠行腹主动脉 下腔静脉分流术。 1 1周后 ,以右心导管法测肺动脉压力 ,观测肺血管显微结构的变化 ,以免疫组织化学方法检测肺动脉hUⅡ的表达。结果 :分流术后 1 1周 ,大鼠肺动脉高压形成 ,肺小血管肌化程度明显增强 ,肺中、小型肌型动脉相对中膜厚度明显增加。分流组大鼠肺动脉内皮细胞和平滑肌细胞hUⅡ蛋白表达上调 ,并且与肺动脉压力和肺血管结构的改变呈正相关。结论 :肺动脉内皮细胞和平滑肌细胞hUⅡ的上调可能参与了高肺血流量所致肺血管结构重建和肺动脉高压的形成。Aim: To study human urotensin Ⅱ (hUⅡ) expression in intrapulmonary arteries of rats with pulmonary hypertension induced by high pulmonary blood flow and explore the role of hUⅡ in the development of pulmonary hypertension induced by left to right shunt. Methods: Aortocaval shunting was produced for 11 weeks in rats. Pulmonary artery mean pressure (PAMP) of each rat was evaluated using right cardiac catheterization. The pulmonary vascular structural changes,including the percentage of muscularized arteries of small pulmonary vessels and relative medial thickness of intra acinar pulmonary arteries were examined. Meanwhile, the expression of hUⅡ by pulmonary arteries was detected by immunohistochemistry. Results: After 11 week aortocaval shunting, PAMP was significantly increased. The percentage of muscularized arteries of small pulmonary vessels and relative medial thickness of pulmonary arteries were obviously increased in shunting rats compared with controls ( P <0.01, respectively). Meanwhile, hUⅡ expression by pulmonary artery endothelial cells and smooth muscle cells was significantly augmented in rats of shunt group, which was positively correlated with PAMP and the structural changes in pulmonary arteries. Conclusion: The up regulation of hUⅡ in pulmonary arteries might be involved in the development of pulmonary vascular structural remodeling and pulmonary hypertension induced by high pulmonary blood flow.

关 键 词：高肺血流 大鼠 肺血管结构 尾加压素Ⅱ 高血压 肺性 肺动脉 

分 类 号：R541.1[医药卫生—心血管疾病]