用固定化弗劳地柠檬酸杆菌XP05从溶液中回收铂  

Recovery of Platinum with Immobilized Citrobacter freudii XP05 Biomass

作　　者：胡洪波[1] 刘月英[1] 傅锦坤[2] 薛茹[2] 古萍英[2] 

机构地区：[1]厦门大学生命科学学院,厦门361005 [2]厦门大学化学化工学院,厦门361005

出　　处：《生物工程学报》2003年第4期456-461,共6页Chinese Journal of Biotechnology

基　　金：国家自然科学基金资助项目 (No .2 98760 2 6)~~

摘　　要：比较了 5种固定弗劳地柠檬酸杆菌XP0 5菌体的方法 ,其中明胶 海藻酸钠包埋法为固定菌体的最佳方法。扫描电子显微镜观察表明 ,XP0 5菌体较均匀地分布于包埋基质中。固定化XP0 5菌体吸附Pt4+ 受吸附时间、固定化菌体浓度、溶液的pH值和Pt4+ 起始浓度的影响。吸附作用是一个快速的过程 ;吸附Pt4+ 的最适pH值为 1 5 ;在 5 0～ 2 5 0mgPt4+ L范围内 ,吸附量与Pt4+ 起始浓度成线性关系 ,吸附过程符合Langmuir和Freundlich吸附等温模型。在Pt4+ 起始浓度 2 5 0mg L、固定化菌体 2 0g L、pH 1 5和 3 0℃条件下 ,振荡吸附 60min ,吸附量为 3 5 3mg g。 0 5mol LHCl能使吸附在固定化菌体上的Pt解吸 98 7%。从废铂催化剂处理液回收铂的结果表明 ,在Pt4+ 起始浓度111 8mg L、固定化菌体 4 0g L、pH 1 5和 3 0℃条件下 ,振荡吸附 60min ,吸附量为 2 0 9mg g。在填充床反应器中 ,在Pt4+ 起始浓度 5 0mg L、流速 1 2ml min、固定化菌体 1 86g的条件下 ,饱和吸附量达 2 4 7mg g;固定化XP0 5菌体经 4次吸附 解吸循环后吸附率仍达 78%。The objective of this work was to develop a valuable adsorbent for recovery of platinum by studying the properties of Pt 4+-adsorption with immobilized Citrobacter freudii XP05 biomass. Five methods for immobilization of Citrobacter freudii XP05 biomass were compared. The method with gelatin-alginate sodium as entrapment matrix was considered to be the optimal. Spherical and uniform beads were produced and the SEM micrograph indicated that the cell of strain XP08 were uniformly dispersed within the matrix. The adsorption of Pt 4+ by immobilized XP05 biomass was affected with adsorptive time, pH value of the solution, immobilized biomass concentration, Pt 4+ initial concentration The adsorption was a rapid process. The optimal pH value for Pt 4+ adsorption was 1 5, and its adsorptive capacity increased linearly with increasing Pt 4+ initial concentrations in the range of 50～250 mg/L. The experimental data could be fitted to Langmuir and Freundlich models of adsorption isotherm. The adsorptive capacity reached 35 2 mg/g under the conditions of 250 Pt 4+ mg/L, 2 0 g/L immobilized biomass, pH 1 5 and 30℃ for 60 min. 98 7% of Pt 4+ adsorbed on immobilized biomass could be desorbed with 0 5 mol HCl/L. The characteristics of dynamic adsorption and desorption of immobilized XP05 biomass in packed-bed reactor were investigated. The saturation uptake was 24 66 mg Pt 4+/g under the conditions of flow rate 1 2 mL/min, pH 1 5, 50 mg Pt 4+/L and 1 85 g biomass(dry weight). Adsorptive efficiency of Pt 4+ by the immobilized XP05 biomass was above 78% for 4 cycles of adsorption and desorption. The recovery of platinum from waste platinum catalyst was studied. The adsorptive capacity was 20 94 mg Pt 4+/g immobilized biomass under the conditions of 4 0 g/L immobilized XP05 biomass, 117 76 mg Pt 4+/L and pH 1 5 for 60 min.The immobilized XP05 biomass is potentially applicable to the recovery of platinum from waste and wastewater containing platinum.

关 键 词：固定化 弗劳地柠檬酸杆菌 回收 铂 生物吸附 

分 类 号：X172[环境科学与工程—环境科学] Q939.9[生物学—微生物学]