急性白血病免疫表型检测及临床观察  

Immunophenotype of acute leukemia and clinical observation

作　　者：迪丽娜孜.阿不来提 温丙昭[1] 李玲[1] 曲建华[1] 钟笛[1] 王蕊[1] 薄蔚[2] 

机构地区：[1]新疆医科大学第一附属医院血液科,新疆乌鲁木齐830054 [2]新疆阿勒泰地区人民医院,新疆阿勒泰836500

出　　处：《新疆医科大学学报》2003年第4期315-317,共3页Journal of Xinjiang Medical University

基　　金：新疆维吾尔自治区科技厅自然科学基金资助项目(2 0 0 12 110 2 )

摘　　要：目的:探讨急性白血病免疫表型特点及其与预后的关系。方法 :用间接免疫荧光法对 10 8例急性白血病行免疫表型检测。 结果 :1初诊按 FAB标准诊断的 M2 、M5和 L3各 1例 ,最后确诊为急性混合细胞性白血病( MAL) ;4例急性未分化型白血病 ( UAL)确诊为急性髓细胞白血病微分化型 ( M0 )。 2 AL L 中 11.5 %伴有髓系抗原表达 ;B- AL L中 CD34 +明显高于 T- AL L (分别为 5 3%和 2 8% ) ,但差异无统计学意义 ( P >0 .0 5 ) ,骨髓内原始 +幼稚细胞数 T- AL L明显低于 B- AL L ( P <0 .0 5 )。 3AML中 2 2例 ( 2 7.8% )伴有至少一个淋系抗原表达。 4CD7+- AML 中 CD34 、CD38、HL A- DR等早期干 /祖细胞抗原高表达 ,CD7在 M3中低表达而在 M0 M1 中高表达 ( P<0 .0 1)。 结论:免疫表型分析对 MAL和 M0 的诊断不可缺少 ;CD7+-Objective: To study the immunophenotype and its relationship with prognosis of acute leukemias. Methods: Immunophenotypes were examined by indirect immunofluorescence method in 108 AL. Results: ① In 108 AL, one case of M\-2, one case of M\-5 and one case of L\-3 diagnosed by FAB morphology, finally rediagnosed as MAL and four cases of UAL were diagnosed as M\-0 by immunophenotyping. ②11.5% cases of ALL coexpressed myeloid associated antigens. CD 34 \++ was most frequently expressed in B ALL than T ALL (53%~28%), but no significant difference was found between two groups(P>0.05). The marrow blast cells were significantly lower in T ALL than B ALL(P<0.05).③ 22 cases (27.8%) of AML coexpressed at least one lymphoid markers. ④ 18 cases expressed CD\-7\++ (22.8%).The early stem cell markers such as CD 34 、 CD 38 、HLA DR were highly expressed in CD\-7\++ AML, CD\-7\++ was rarely expressed in M\-3 but highly expressed in M\-0 M\-1. Conclusion: There was significant value of immunophenotyping for the diagnosis of MAL and M\-0. The expression of CD\-7 in AML might predicts worse prognosis.

关 键 词：急性白血病 免疫分型 预后 

分 类 号：R733.71[医药卫生—肿瘤] R730.3[医药卫生—临床医学]