一种改良的对虾白斑综合征病毒的提纯技术  

A Modified Technique for Purifying White spot syndrome virus

作　　者：解云礼[1] 张叔勇[1] 黄茹[1] 石正丽[1] 

机构地区：[1]中国科学院武汉病毒研究所分子病毒学重点实验室无脊椎动物病毒学联合开放实验室,湖北武汉430071

出　　处：《中国病毒学》2003年第4期391-393,共3页Virologica Sinica

基　　金：中国科学院生命科学与生物技术领域2001年知识创新工程重要方向项目(KSCX2-SW-302-2);863海洋生物技术课题(2001AA620201)

摘　　要：对虾白斑综合征病毒(White spot syndrome virus,WSSV)是对虾养殖业的主要病原,自1992年以来一直严重影响对虾的产量和质量,造成巨大的经济损失.The envelope proteins of White spot syndrome virus (WSSV) are very fragile and easy to be destroyed during purification. It was difficult to obtain a large quantity of intact virions by routine sucrose gradient centrifugation. After modifying the sucrose gradient by adding citrate sodium, we can obtain a large quantity of intact virions and nucleocapsids. This purified virions and nucleocapsids were subsequently used for analyzing viral structural proteins and DNA extraction. The result showed that this modified technique is very efficient for virus purification.

关 键 词：对虾白斑综合征病毒 提纯 病毒粒子 病毒囊膜 

分 类 号：S945[农业科学—水产养殖] Q939.4[农业科学—水产科学]