伪狂犬病病毒Min-A株gE基因的克隆及序列分析  

Cloning and sequence analysis of the gE gene of pseudorabies virus Min-A strain

作　　者：胡涛[1] 崔保安[1] 王学斌[1] 杨明凡[1] 张素梅[1] 王岩 

机构地区：[1]河南农业大学畜牧兽医工程学院,河南郑州450002 [2]郑州牧业高等专科学校,河南郑州450008

出　　处：《中国兽医科技》2003年第8期15-19,共5页Chinese Journal of Veterinary Science and Technology

摘　　要：参考GenBank中伪狂犬病病毒 (PRV) gE基因的序列设计了 1对引物 ,对PRVMin A株进行了PCR扩增 ,扩增产物克隆于 pGEM TEasy载体。对重组质粒进行限制性内切酶分析和基因测序 ,证实了克隆片段的可靠性。测序结果表明 ,目的片段包含 1个 174 0bp的开放性阅读框(ORF) ,编码由 5 79个氨基酸组成的多肽。同源性分析表明 ,PRVMin A株与PRVEa株、SH株gE基因的核苷酸同源性分别为 99.2 %、98.7% ;推导氨基酸的同源性分别为 98.6 %、97.2 %。A pair of primers was designed according to the seq ue nces pulished by the GenBank in order to amplify gE gene of the pseudorabies virus Min-A strain.The gE gene was obtained by pol ymerase chain reaction, and then cloned into the pGEM-T Easy vector.The recombinant plasmid of pGTE-E was identified by restriction enzyme analysis an d sequencing ,Which proved completely its validity.Nucleotide sequenci ng revealed that this fragment contained an open reading frame of 1 740 bp en coding a 579 aa protein . The homology of the nucleotide sequence of PRV Min -A strain gE with PRV Ea strain,SH strain was 99.2% and 98.7%,respectively ;and the homology of predicted amino acids among them was 98.6% and 97.2%,respectiv ely.

关 键 词：伪狂犬病病毒 Min—A株 GE基因 基因克隆 序列分析 伪狂犬病 

分 类 号：S852.659.1[农业科学—基础兽医学]