细菌视紫红质能化态时表面电位的非线性光学机制  

Study on Nonlinear Optical Mechanism of Surface Potential in Energized Bacteriorhodopsin

作　　者：黄燕萍[1] 沈珊雄[1] 李琳[1] 李珊珊[1] 黄立 李庆国[2] 

机构地区：[1]华东师范大学物理系光谱学和波谱学教育部重点实验室,上海200062 [2]复旦大学生物物理系,上海200433

出　　处：《光学学报》2003年第8期902-905,共4页Acta Optica Sinica

基　　金：上海市自然科学基金 (0 1ZF14 0 17);上海市重点学科资助课题

摘　　要：用荧光标记物 1,8 ANS与细菌视紫红质结合 ,测得紫膜细菌视紫红质在能化态时的表面电位远大于非能化态时的对应值。在细菌视紫红质分子的激光四波混频实验中 ,应用激子表象理论 ,获得了紫膜能化态时的激子饱和密度和激子长度 ,说明在bR ANS络合物中 ,细菌视紫红质中色氨酸残基对ANS的激发能量转移效率提高 ,能化态时表面电荷密度增加 ,从而使非辐射共振转移变为激子转移 ,也证明了紫膜能化态时表面电位的非线性光学机制。The effects of surface potential on the binding and fluorescence of 1 anilinonaphthalene 8 sulfonate (ANS) in suspensions of bacteriorhodopsin are investigated. The ANS binding depends on the surface potential which modulates the apparent binding constant of ANS on the bR membrane. The surface potential of the binding ANS measured in energized is larger than in nonenergized bR membrane. In the laser four wave mixing experiment, the saturation density and the length of exitons are obtained under the one\|dimensional exciton phase space filling model. The values result in improving the highly efficient energy transfer from the aromatic amino acids of bR to the bound ANS and in raising the density of the surface charge of the energized bR so that the non radiation resonant transfer is made to be exciton transfer, and the surface potential of energized bR has its nonlinear optical mechanism.

关 键 词：细菌视紫红质 能化态 表面电位 非线性光学机制 紫膜 激光四波混频实验 蛋白质分子 

分 类 号：Q51[生物学—生物化学] Q63