氟化物对体外器官培养人牙胚细胞增殖及相关调控蛋白表达的影响  

Effect of fluoride on human tooth-germ cell proliferation and expression of related regulators in organ culture in vitro

作　　者：侯铁舟[1] 李志丹[1] 王强[1] 李相如[1] 王红[1] 陶洪[1] 

机构地区：[1]西安交通大学口腔医学院口腔内科,陕西西安710004

出　　处：《西安交通大学学报（医学版）》2003年第4期302-305,共4页Journal of Xi’an Jiaotong University（Medical Sciences）

基　　金：陕西省科技攻关基金资助项目 (No .2 0 0 1K1 0 G9) ;西安交通大学重点科研培植项目 (No.PZ0 98)

摘　　要：目的　观察氟对牙胚细胞增殖及相关调控蛋白表达的影响。方法　采用人牙胚体外器官培养模型 ,组织学观察低浓度 (10mg·L- 1 )和高浓度 (2 5mg·L- 1 )氟对细胞增殖的影响 ;免疫组织化学及图像分析技术检测氟对细胞周期蛋白D1 (cyclinD1 )和细胞增殖核抗原 (PCNA)表达的影响。结果　氟处理组细胞增殖受抑 ,cyclinD1 和PCNA的表达显著降低 (P <0 .0 1)。结论　氟化物可能通过抑制cyclinD1 和PCNA的表达引起细胞增殖抑制 。Objective To investigate the effect of fluoride on human tooth-germ cell proliferation and expression of related regulators. Methods By using an organ culture model of human tooth-germ in vitro, the effects of low (10?mg·L -1) and high (25?mg·L -1) concentrations of fluoride on cell proliferation were observed histologically and the expression of cyclinD 1 and proliferating cell nuclear antigen (PCNA) were detected by immunohistochemistry staining and image analysis technique. Results Fluoride inhibited cell proliferation and evidently decreased the expression of cyclinD 1 and PCNA. Conclusion Fluoride may inhibit tooth-germ cell proliferation by inhibiting the expression of cyclinD 1 and PCNA, which leads to the abnormal development of teeth.

关 键 词：氟化物 器官培养 人牙胚细胞增殖 细胞周期蛋白D1 细胞增殖核抗原 龋病 氟斑牙 

分 类 号：R781[医药卫生—口腔医学] R329.2[医药卫生—临床医学]