SARS冠状病毒基因片段变异分析  

Analysis of Variations in Gene Fragments of SARS-CoV

作　　者：王义权[1] 姚冠华 牛建军 黄磊[1] 纪念念[1] 李奇渊[1] 黄建炜 邵寒娟[1] 陈亮[1] 章军[1] 宋思扬[1] 彭宣宪[1] 

机构地区：[1]厦门大学生命科学学院,细胞生物学与肿瘤细胞工程教育部重点实验室,福建厦门361005 [2]厦门市卫生防疫站,福建厦门361004

出　　处：《厦门大学学报（自然科学版）》2003年第5期545-547,共3页Journal of Xiamen University：Natural Science

基　　金：厦门市科技计划资助课题

摘　　要：采用基因序列和生物信息分析法,研究了SARS CoV复制酶基因中4个片段.结果发现,片段I与已报道的SARS冠状病毒ZJ01有2个位点不同,片段III与CUHK W1、CUHK Su10、HKU 39849和Hong Kong各有1个位点的差别;片段IV与GZ01间有1个位点的变化.同时对已公布的17个全基因组序列进行比对分析,可找到共137个变异位点,其中仅出现1次的变异位点119个,间约信息位点18个.Four gene fragments from replication enzyme gene of SARSCoV,namely fragment I ,fragment II,fragment III and fragment IV,were investigated with combination of sequence analysis and bioinformatics.Our results showed that there were several variations existing in three of the four fragments, showing that fragment I had two variance against GZ01,fragment III had 1 nuclear acid change comparing with CUHKW1,CUHKSu10, HKU39849 and HongKong respectively,and fragment IV had 1 point mutation against GZ01.In addition,alignment analysis of 17 complete sequences downloaded from GeneBank revealed 137 variable sites,of which 18 sites were of parsimony informative.Our findings are valuable for further study on variations of SARSCoV.

关 键 词：急性严重呼吸道综合征 SARS冠状病毒 基因片段 基因变异 变异位点 基因序列分析 

分 类 号：R373.1[医药卫生—病原生物学] Q754[医药卫生—基础医学]