海洋细菌1202菌株产胞外多糖的适宜条件  

Suitable Culture Conditions for Exopolysaccharides Produced by Marine Bacterial Strain 1202

作　　者：郑忠辉[1] 刘三震[1] 黄益丽[1] 黄耀坚[1] 宋思扬[1] 苏文金[1] 

机构地区：[1]厦门大学生命科学学院,福建厦门361005

出　　处：《厦门大学学报（自然科学版）》2003年第5期661-664,共4页Journal of Xiamen University：Natural Science

基　　金：国家"863"计划(2001AA620401);国家自然2100.651.30.25科学基金(30070024)资助

摘　　要：应用响应面法(ResponseSurfaceMethod,RSM)对海洋细菌1202菌株胞外多糖发酵培养基进行优化.结果表明,适宜的发酵培养基组成为:蔗糖4%,酵母膏0.05%,玉米浆1.5%,CaCO30.15%,KH2PO40.08%,MgSO4·7H2O0.05%,FeSO4·7H2O0.01%,NaCl0.1%,pH7.5.在装液量为50mL(500mL三角瓶),接种量5%时,于28℃摇瓶培养7d,胞外多糖的产量可达到8.3mg/mL,转化率为20.75%.The response surface method (RSM) was used to optimize the medium for production of exopolysaccharides by marine bacterium 1202. The optimal fermentation conditions were established with 5% inoculum size and 500 mL Erlenmeyer flask containing 50 mL medium, which consisted of 4% sucrose,1.5% corn steep liquor,0.05% yeast extract,0.15% CaCO3,0.05% MgSO4·7H2O,0.08% KH2PO4,0.01% FeSO4·7H2O,0.1% NaCl,pH7.5.The fermentation was carried out at 28℃ for 7 days on a rotary shaker.Under these conditions, the yield of exopolysaccharide reached 8.3 g/L,and the conversion rate of sucrose was 20.75%.

关 键 词：海洋细菌 12O2菌株 胞外多糖 发酵培养基 适宜条件 响应面法 免疫调节活性 药理作用 

分 类 号：TQ464.1[化学工程—制药化工] TQ460.38