无机盐和碳氮源对青霉PT95类胡萝卜素产率的影响  

EFFECT OF VARIOUS INORGANIC SALTS, CARBON AND NITROGEN SOURCES ON CAROTENOID YIELD IN SURFACE CULTURES OF PENICILLIUM SP.PT95

作　　者：徐军[1] 崔丽霞[1] 韩建荣[1] 

机构地区：[1]山西大学生命科学系,太原030006

出　　处：《微生物学通报》2003年第4期77-81,共5页Microbiology China

基　　金：国家自然科学基金资助项目 (No 30 0 70 0 2 1)~~

摘　　要：供试的 4种无机盐中 ,K2 HPO4的单因子效应最好 ;K2 HPO4+KCl +MgSO4表现出最好的正协同效应。 5种碳源都能被PT95菌株利用 ,麦芽糖和蔗糖是最适碳源。在以酵母膏为氮源的培养基上 ,PT95菌株的菌核生物量最高 ;而在以蛋白胨为氮源的培养基上 ,类胡萝卜素产率最高 ;铵盐和尿素对菌核形成不利 ;硝酸钠是最好的无机氮源。培养基中的含氮量保持在 0 2 4～ 0 48g L,含碳量保持在 5 2 6～ 2 1 0 5g L,有利于PT95菌株形成菌核和积累色素 ;培养基的最适C N比为 2 5∶1。Among inorganic salts tested, K 2HPO 4 was more essential to the sclerotia for mation and carotenogenesis of strain PT95 than KCl, MgSO 4 or FeSO 4 It wa s also shown that the combination of K 2HPO 4, KCl and MgSO 4 could produce t he best positive cooperation and give the highest sclerotia biomass (782mg/plate ) and pigment yield (328 μg/plate) Five carbon sources, i e glucose sucros e, lactose, maltose and soluble starch, all could be utilized by the strain PT95 , and maltose was the best Among 8 nitrogen sources, yeast extract favooured t he sclerotia formation, and peptone fovoured the pigment accumulation; amine sal ts and urea were unfavourable to form sclerotia The medium containing 0 24 ～ 0 48 g/L sodium nitrate nitrogen was effective to both the sclerotia formation and the carotenoid production of strain PT95 when available maltose carbon con centrations were at 5 26～21 05 g/L The optimal C/N ratio was found to be 25 ∶1

关 键 词：青霉 无机盐 碳源和氮源 类胡萝卜素产率 菌核生物量 

分 类 号：Q939.9[生物学—微生物学]