链霉素抗性突变——纳他霉素高产菌株的选育研究  

STREPTOMYCIN RESISTANCE MUTATION——A STUDY ON THE BREEDING OF HIGH NATAMYCIN-PRODUCING STRAIN

作　　者：杨东靖[1] 陈冠群[1] 陈巍[1] 王敏[1] 杜连祥[1] 

机构地区：[1]天津科技大学食品科学与生物工程学院,天津300222

出　　处：《微生物学通报》2003年第4期29-32,共4页Microbiology China

基　　金：天津市自然科学基金资助项目 (No 0 136 0 9511)

摘　　要：应用链霉素抗性筛选法 ,将经过紫外线诱变处理的纳他霉素生产菌———褐黄孢链霉菌 (Streptomycesgilvosporeus)ATCC1 332 6的孢子涂布在含有链霉素最小抑制浓度 (0 6μg mL)的培养基平板上 ,获得了 1 2 2株链霉素抗性突变株。其中纳他霉素产量高于出发菌株的有1 3株 ,产量阳性效率达到 1 0 6 % ,同时获得了产抗生素能力为出发菌株 1 46倍的突变株SG 56。A method of streptomycin resistance screening was applied to improve t he productivity of Natamycin by Streptomyces gilvosporeus(ATCC13326) The sp ores treated with UV light were regenerated on agar plates containing 0 6μg/mL stre p tomycin 122 streptomycin resistant(str) mutants were obtained The Natamycin y iel ds of 13 mutants were higher than the original strain The mutants with high Na t amycin productivity were screened at a high frequency(10 6%) The highest one that demonstrated 1 46 times that of the original strain in Natamy cin productivity was obtained

关 键 词：褐黄孢链霉菌 链霉素抗性筛选法 诱变 纳他霉素 

分 类 号：Q933[生物学—微生物学]