EB病毒潜伏膜蛋白1在鼻咽癌细胞中通过ERK介导Ets-1表达  

Epstein-Barr Virus Latent Membrane Protein1(LMP1) Activated Ets-1 Expression Mediated by ERK in Nasopharyngeal Cell Line

作　　者：曾亮[1] 李敏 宋鑫[1] 陶永光[1] 唐敏[1] 李嵬[1] 曹亚[1] 

机构地区：[1]中南大学湘雅医学院肿瘤研究所,长沙410078 [2]基因公司长沙分公司,长沙410011

出　　处：《生物化学与生物物理进展》2003年第4期574-578,共5页Progress In Biochemistry and Biophysics

基　　金：国家重点基础研究发展规划项目 (973 )"鼻咽癌发生发展的基础研究" (G19980 5 12 0 1);国家重点基础研究发展规划项目 (973 )"恶性肿瘤侵袭和转移的机理及分子阻遏" (2 0 0 2CB5 13 10 0 )~~

摘　　要：为了探讨EB病毒编码的潜伏膜蛋白 1(LMP1)对核转录因子Ets 1表达和活化的影响 ,并证实细胞外信号调节激酶 1/ 2 (ERK1/ 2 )参与了该过程 ,选用可调控表达LMP1的鼻咽癌细胞系L7,应用蛋白质印迹法检测Ets 1、p ERK蛋白质表达 ,免疫共沉淀 蛋白质印迹法检测Ets 1磷酸化状态 ,使用ERK1/ 2特异性小分子阻断物PD980 5 9作用后 ,蛋白质印迹法检测 p ERK、Ets 1表达及磷酸化变化 .结果显示 :在L7细胞中诱导性LMP1可促进p ERK、Ets 1蛋白质表达及其苏氨酸残基磷酸化 ,在一定范围呈时间和剂量效应 ;通过PD980 5 9对诱导性LMP1作用的干预发现 ,p ERK大部分表达被阻断 ,而Ets 1表达及其苏氨酸磷酸化也被部分阻断 ,以上结果提示ERK部分介导了LMP1诱导EtsTo confirm the effect of EBV encoded LMP1 on expression and activation of nuclear transcription factor, Ets-1 and extracellualr signal regulated kinase (ERK) involved in the process. The expression of p-ERK and Ets-1 were assayed with Western blot, and phosphorylation of Ets-1 was assayed with co-immunoprecipitation-Western blot. MEK1/ERK specific inhibitor PD98059 was used to confirm that ERK mediated the activation of Ets-1 by LMP1. The results showed that in nasopharyngeal carcinoma cell line, EBV-LMP1 enhanced the expression of Ets-1 and p-ERK, and phosphorylation of Ets-1 to some extent in time- and dose-dependent manner. With blockade of PD98059, expression of p-ERK induced by LMP1 decreased significantly, and expression and phosphorylation of Ets-1 by LMP1 decreased partly. These results suggest that expression and phosphorylation of Ets-1 by LMP1 was mediated partly by ERK.

关 键 词：EB病毒 潜伏膜蛋白1 鼻咽癌细胞 ERK介导 胞外信号调节激酶 

分 类 号：R739.6[医药卫生—肿瘤]