肺癌转移相关蛋白的比较蛋白质组分析与鉴定  

Characterization and Identification of Metastasis-associated Proteins of Lung Cancer by Comparative Proteome Analysis

作　　者：蒋代凤[1] 应万涛[1] 万晶宏[1] 邱宗荫[2] 钱小红[1] 贺福初[1] 

机构地区：[1]军事医学科学院放射医学研究所基因组学与蛋白质组学实验室,北京100850 [2]重庆医科大学检验系,重庆400016

出　　处：《生物化学与生物物理进展》2003年第4期586-593,共8页Progress In Biochemistry and Biophysics

基　　金：国家重点基础研究资助项目 (2 0 0 1CB5 10 2 0 4;2 0 0 1CB5 10 2 0 1;G19980 5 12 13 );国家自然科学基金重大项目 (3 973 0 2 70 ;3 9990 60 0 )部分资助~~

摘　　要：采用比较蛋白质组技术对肺巨细胞癌高、低转移株的蛋白质表达谱进行双向电泳分离和MALDI TOF分析 ,并在蛋白质和mRNA水平进行进一步验证 ,成功鉴定了 11个肺癌转移相关蛋白 .其中 ,候选蛋白膜联蛋白1(ANX1)、细胞骨架蛋白 (CK18)、Rho GDP解离抑制剂 1(GDIR)、原肌球蛋白 3(TPMF)、蛋白谷氨酰胺γ 谷氨酰转移酶 (TGLC)和白介素 18(IL 18)在肺巨细胞癌高转移株显著高表达 ,而候选蛋白核氯离子通道蛋白 1(CLI1)、蛋白质二硫键异构酶 (ER6 0 )、肌酸激酶、硫氧还蛋白过氧化物酶 1(PDX1)和热休克蛋白 6 0(CH6 0 )在肺巨细胞癌高转移株显著低表达 .大多数的候选蛋白可通过调控肿瘤细胞的生长、迁移、粘附、凋亡和肿瘤免疫等环节来影响肿瘤细胞的侵袭和转移能力 .迄今为止 ,还未见候选蛋白CLI1和IL 18与肺癌转移相关的报道 。Comparative proteomic analysis, combined with 2-DE separation and MALDI-TOF identification, was utilized to compare the protein expression profiles between highly and lowly metastatic subpopulations (I. e. PLA801D and PLA801C) and 11 metastasis-associated proteins were identified and further validated by I-D Western blotting, Northern blot and/or semi-quantitative RT-PCR analysis. Compared with that in lowly metastatic subpopulation, CK18, TGLC, GDIR, TPMF, IL-18 and ANX1 were significantly up-regulated, while ER60, CH60, PDX1, CLI1 and KCRB were significantly downregulated in highly metatsatic subpopulation. Most of the candidate proteins have been evidenced to be somehow associated with various aspects of tumor metastasis such as cell growth, motility, invasion, adhesion. apoptosis and tumor immunity, etc. Up to now, CL11 and IL-18 have not been reported to be associated with lung metastasis, which implied that CL11 and IL-18 might be new metastasis-associated proteins of lung cancer.

关 键 词：肺癌 转移 蛋白质组 核氯离子通道蛋白1 白介素18 

分 类 号：R734.2[医药卫生—肿瘤]