冻干家兔眼角膜复水工艺及角膜全层检测  

Hydration and Full Layers Examination of Freeze-dried Rabbit Corneas

作　　者：王德喜[1] 徐成海[1] 张世伟[1] 陈晓隆[2] 曹永梅[2] 

机构地区：[1]东北大学机械工程及自动化学院 [2]中国医科大学第二临床医院,沈阳110003

出　　处：《真空科学与技术》2003年第4期278-281,共4页Vacuum Science and Technology

基　　金：国家自然科学基金资助 (No.59876 0 0 8)

摘　　要：角膜的真空冷冻干燥是离体生物组织的冻干 ,目的是便于贮藏 ,以备后用。冻干角膜的复水是决定角膜活性的关键环节。本文以冻干的兔角膜为实验材料 ,对其复水进行研究 ,找到了合适的复水工艺。对按该工艺复水的冻干角膜进行了全层透射电镜检测 ,结果表明冻干角膜的上皮细胞层、前弹力膜、基质层。A novel technique has been successfully developed to hydrate freeze dried rabbit corneas.The hydrated rabit cornea was examined layer by layer with transmission electron microscopy(TEM) to evaluate its organic structures.As compared with TEM images of the control sample,a fresh rabbit cornea,no abnormal observation was found showing possible damage of the organic structures in both the layer connections and the five layers,including epithelial layer,bowman membrane,stroma,descemet membrane and endothelium layer.

关 键 词：角膜 真空冷冻干燥 复水 全层检测 

分 类 号：Q68[生物学—生物物理学] TK124[动力工程及工程热物理—工程热物理]