尿本周氏蛋白及转化生长因子β_1对肾近端小管上皮细胞增殖的影响  

Effects of Bene Jones protein and TGF-β_1 on the proliferation of rat renal proximal tubular cells in vitro

作　　者：周振海[1] 李幼姬[1] 陈运贤[2] 李小银[1] 余学清[1] 李娟[2] 罗绍凯[2] 原耀光[2] 

机构地区：[1]中山大学附属第一医院肾内科,广东广州510080 [2]中山大学附属第一医院血液内科,广东广州510080

出　　处：《中国病理生理杂志》2003年第9期1257-1260,共4页Chinese Journal of Pathophysiology

摘　　要：目的 :研究多发性骨髓瘤 (MM)患者尿本周氏蛋白 (BJP)及转化生长因子 β1(TGF - β1)在体外对肾近端小管上皮细胞 (PTC)增殖的影响。方法 :将自MM患者尿中提取的λ型BJP和 /或TGF - β1与大鼠NRK .5 2E株PTC共同培养 ,用 [3 H]TdR掺入研究PTC增殖。并用ELISA法测定BJP与PTC培养上清液中的TGF - β1含量。结果 :①10 0 - 80 0 μmol/LBJP以浓度依赖方式抑制PTC增殖 ,当浓度≥ 4 0 0 μmol/L与对照组比较有显著差异 (P <0 .0 5 ) ,当加入 2 μg/LTGF - β1共同培养 ,在BJP浓度为 4 0 0 μmol/L时 ,[3 H]TdR掺入低于单用BJP(P <0 .0 5 ) ,但与单用BJP浓度为 80 0 μmol/L无显著差异 ;② 10 0 - 80 0 μmol/LBJP与PTC共同培养液中的TGF - β1含量均增加 ,尤以BJP为 4 0 0μmol/L明显大于对照组 (P <0 .0 5 ) ;③外源性 0 .5 - 10 μg/LTGF - β1与PTC共同培养 ,[3 H]TdR掺入随TGF - β1浓度增加而减少。结论 :MM患者λ型BJP可抑制PTC的增殖 ,其部分机理可能与其促进对PTC增殖也有抑制作用的TGF-AIM: To study the effect of Bene Jones protein (BJP) from multiple myeloma(MM) patient and TGF-β 1 on cultured renal proximal tubular cell(PTC) proliferation. METHODS:[ 3H] TdR incorporation was used to study the effect of λBJP and TGF-β 1 on cultured rat NRK.52E PTC proliferation,the expression of TGF-β 1 in the supernatant of PTC cultured with BJP was assessed with ELISA. RESULTS:① [ 3H] TdR incorporation of PTC was inhibited by BJP in a dose-dependent manner,when co-cultured with 100-800 μmol/L BJP and 2.0 μg/L TGF-β 1, the [ 3H] TdR incorporation was lower than that of BJP alone, especially when BJP≥400 μmol/L; ②The expression of TGF-β 1 in the supernatant of PTC cultured with BJP was increased ,especially when BJP≥400 μmol/L( P< 0.05); ③ The [ 3H] TdR incorporation of PTC was also inhibited by exogenous TGF-β 1 in a dose-dependent manner. CONCLUSION: λBJP has antiproliferative effect on rat PTC in vitro , The effect is related with stimulating the PTC to produce excessive TGF-β 1, which also has antiproliferative effect on PTC in some degree.

关 键 词：本周蛋白 转化生长因子Β 近端肾小管上皮细胞 

分 类 号：R363[医药卫生—病理学]