葛根素对慢性低氧高二氧化碳大鼠肺动脉管壁胶原代谢的影响  

Effect of puerarin on pulmonary arterioles wall collagen metabolism of chronic hypoxia hypercapnia rats

作　　者：龚永生[1] 李继武[1] 杨鹏麟[1] 范小芳[1] 胡良冈[1] 郑绿珍[1] 蒋仲荪[1] 

机构地区：[1]温州医学院肺心病研究室附属第二医院心内科,浙江温州325027

出　　处：《中国病理生理杂志》2003年第9期1261-1264,T008,共5页Chinese Journal of Pathophysiology

摘　　要：目的 :探讨葛根素对慢性低氧高二氧化碳大鼠肺动脉管壁胶原代谢的影响。方法 :采用图像分析、氯胺T法、免疫组化、组织原位杂交技术等方法观察葛根素对慢性低氧高二氧化碳大鼠肺组织羟脯氨酸含量、肺小血管显微结构、肺动脉管壁Ⅰ、Ⅲ型胶原和Ⅰ、Ⅲ型前胶原基因的影响。结果 :①光镜下葛根素组内弹力板扭曲、中膜平滑肌细胞增生及管腔狭窄程度均明显轻于低O2 高CO2 组。②血浆ET含量低O2 高CO2 组明显高于正常对照组(P <0 0 1) ,葛根素组明显低于低O2 高CO2 组 (P <0 0 1)。血浆NO含量低O2 高CO2 组明显低于正常对照组 (P <0 0 1) ,葛根素明显高于低O2 高CO2 组 (P <0 0 1)。③氯胺T法发现肺组织羟脯氨酸含量低O2 高CO2 组明显高于正常对照组 (P <0 0 1) ,葛根素组明显低于低O2 高CO2 组 (P <0 0 1)。④免疫组化、原位杂交法发现肺细小动脉Ⅰ型胶原和Ⅰ型前胶原mRNA平均吸光度值低O2 高CO2 组明显高于正常对照组 (P <0 0 1) ,葛根素组明显低于低O2 高CO2 组 (P <0 0 1)。而Ⅲ型胶原及Ⅲ型前胶原mRNA平均吸光度值各组间无显著差异 (P >0 0 5 )。结论 :葛根素降低慢性低O2 高CO2 性肺动脉高压、改善肺血管重建可能与其抑制肺动脉管壁胶原的沉积有关。AIM:To investigate the effect of puerarin on pulmonary vessel collagen metabolism in pulmonary hypertension rats induced by chronic hypoxia and hypercapnia. METHODS: Collagen Ⅰ,Ⅲ and their mRNA were observed in pulmonary arterioles by the technique of immunohistochemistry and in situ hybridization. RESULTS: ① Light microscopy showed media thickness of pulmonary arterioles was much higher in HH(hypoxic-hypercapnia) group than that of NC(normal control) group, and, vessel cavity turned more straiter in HH group than that of NC group.However, the damage of pulmonary arterioles in HP(hypoxic-pueratin) group was much slighter than that of HH group. ② The levels of plasma ET-1 and lung homogenates Hyr were much higher in HH group than those of NC group( P< 0.01), and lower in HP group than HH groups( P< 0.01).Plasma NO content in group HH was lower than that of group NC( P< 0.01),it was higher in group HP than that of group HH( P< 0.01).③Expression of collagen Ⅰ and collagen Ⅰ mRNA in pulmonary arterioles were significantly higher in HH groups than those of NC group ( P< 0.01),and they were lower in HP group than those of HH group ( P< 0.01).Expression of collagen Ⅲ and collagen Ⅲ mRNA showed no difference among three groups( P> 0.05). CONCLUSION: Puerarin inhibited the deposition of collagen and improved pulmonary vessel remodeling.

关 键 词：葛根素 低氧 慢性 高碳酸血 高血压 肺性 胶原 

分 类 号：R363[医药卫生—病理学]