低O_2高CO_2对大鼠肺中小动脉可溶性鸟苷酸环化酶基因及其蛋白表达的影响  

Effects of hypoxia and hypercapnia on expression of soluble guanylate cyclase in rat pulmonary artery

作　　者：夏晓东[1] 徐正衸[2] 王群姬[3] 张洪勤[4] 

机构地区：[1]温州医学院附属第二医院呼吸内科,浙江温州325027 [2]温州医学院病理生理教研室,浙江温州325027 [3]温州医学院附一院病理科,浙江温州325027 [4]温州医学院分子生物学中心,浙江温州325027

出　　处：《中国病理生理杂志》2003年第9期1214-1216,T004,共4页Chinese Journal of Pathophysiology

基　　金：浙江省自然科学基金资助项目 (No .396 4 79)

摘　　要：目的 :探讨慢性低O2 高CO2 时大鼠肺动脉可溶性鸟苷酸环化酶 (sGC)基因及其蛋白表达的变化。方法 :复制大鼠低O2 高CO2 肺动脉高压模型 ,免疫组织化学技术观察低O2 高CO2 肺动脉高压 1、2、4周组及对照组肺中小动脉sGCα1、β1亚基蛋白的表达 ,原位杂交技术观察肺中小动脉sGCα1亚基mRNA的表达。结果 :低O2 高CO2 1、2、4周组肺中小动脉sGCα1、β1亚基蛋白及α1亚基mRNA表达弱于对照组 (均P <0 .0 1)。结论 :低O2 高CO2 抑制肺中小动脉sGCmRNA及其蛋白的表达 。AIM: To investigate the expression of soluble guanylate cyclase protein and its mRNA in rat pulmonary artery after exposure to hypoxia and hypercapnia. METHODS: Male Sprague-Dawley rats were randomly split into 4 group, which were hypoxic hypercapnic (HH 1 week, HH 2 weeks, HH 4 weeks) group and control group, to copy pulmonary hypertensive animal model. The expression of sGCα 1 and β 1 subunits protein of medial and small pulmonary artery was performed by immunohistochemistry with a polycolonal antibody. In situ hybridization was performed on the rat lung tissue using sGC oligonuclear probe to assay the expression of sGCα 1 subunit mRNA. RESULTS: The sGCα 1 and β 1 subunits protein and sGCα 1 subunit mRNA were faint staining in the pulmonary small and medium artery in HH1 week, HH 2 weeks and HH 4 weeks groups compared with control group (all P< 0.01). CONCLUSION: sGC subunit mRNA and protein expression in pulmonary small and medium artery were decreased after exposure to hypoxia and hypercapnia, which took part in the development of the pulmonary hypertension.

关 键 词：低氧 高碳酸血症 肺动脉 可溶性鸟苷酸环化酶 基因 

分 类 号：R363[医药卫生—病理学]