DNA共价结合在化学修饰云母片上的AFM研究  

AFM Studies on DNA Covalently Immobilized on Mica Modified by APTES-GA

作　　者：祭美菊[1] 侯鹏[1] 沈佳尧[1] 李松[1] 陆祖宏[1] 何农跃[1] 

机构地区：[1]东南大学教育部分子与生物分子电子学开放实验室,南京210096

出　　处：《高等学校化学学报》2003年第9期1621-1623,共3页Chemical Journal of Chinese Universities

基　　金：国家重大基础研究项目(批准号:G1998051204);国家自然科学基金(批准号:60071001)

摘　　要：原子力显微镜（AFM）自1986年发明以来, 已经成为生物学研究领域中的一个有效工具，尤其在核酸及其它生物大分子结构方面的应用已成为普遍关注的热点.The immobilization of DNA on self-assembled layers of 3-aminopropyltrimethoxysilane (APTES) and glutaraldehyde(GA) on mica wafers by chemical coupling has been studied by using atomic force microscopy (AFM). The DNA used for our investigation is amplified by PCR and primers were labeled by -NH2 at their 5' terminal. The surfaces were analyzed with X-ray photoelectron spectroscopy (XPS) and AFM. The results from XPS and AFM show that APTES-GA modified mica can be formed and the flatness of mica is adapted to AFM images. We found that DNA can be readily immobilized on mica. The covalent coupling between the aldehyde-terminated membrane and the both H2N-groups in double strands DNA results in the immobilization of the DNA straightly.

关 键 词：DNA 共价偶联 化学修饰 云母片 AFM 脱氧核糖核酸 原子力显微镜 生物学研究 蛋白质 

分 类 号：Q523[生物学—生物化学] Q51