刺梨主要基因型的RAPD鉴别  

Identification of genotypes of cili as revealed by randomly amplified polymorphic DNA

作　　者：文晓鹏 邓秀新[1] 樊卫国[2] 

机构地区：[1]华中农业大学作物遗传改良国家重点实验室,湖北武汉410070 [2]贵州大学农学院,贵州贵阳550025

出　　处：《山地农业生物学报》2003年第4期317-321,共5页Journal of Mountain Agriculture and Biology

基　　金：国家自然科学基金(39830260);贵州省自然科学基金(20013031)

摘　　要：采用RAPD分子标记,构建了刺梨8个基因型的DNA指纹图谱和亲缘关系图。结果表明,以筛选出的16个随机引物进行PCR扩增,多态性检出率高,其中以OPB-11、OPW-02和OPAF-16多态性最好,能有效地用于供试材料的鉴别;基因型之间多态性百分率(PP)差异较大,以重瓣刺梨最高(60.9％),无籽刺梨最低(48.3％);通过RAPD遗传距离矩阵,采用UPGMA法构建了8个基因型的聚类图,并结合多态性百分率,分析了基因型之间,特别是重瓣刺梨、白花刺梨及无籽刺梨与5个栽培类型间的亲缘关系。To discriminate the 7 genotypes from Rosa roxburghii Tratt and 1 genotype of R. sterilis S. D. Shi,and investigate their genetic relationships, RAPD technique using 16 screened arbitrary primers were carried out. The results showed that.OPB -11 ,OPW -02 and OPAF - 16 were the most polymorphic primers in fingerprinting to the given genotypes; and their UPGMA dendrogram based on genetic distance matrix were constructed, the relatedness between R. roxburghii Tratt f. roxburghii,R. roxburghii Tratt f. Candida,and R. sterilis and 5 cultivated genotypes from Rosa roxburghii Tratt were discussed. In addition, their percentages of polymorphism were analyzed, which R. roxburghii Tratt f. roxburghii showed the highest value(60. 9% ) ,while R. sterilis was the least polymorphic(48. 3% ).

关 键 词：刺梨 基因型 RAPD分子标记 鉴别 栽培类型 亲缘关系 聚类分析 

分 类 号：S661.2[农业科学—果树学]