应用RT-PCR检测乳腺癌患者外周血CK-19mRNA的临床意义  

Detection of CK-19 mRNA in peripheral blood from breast cancer patients by reverse transcription polymerase chain reaction

作　　者：关泉林[1] 谭继英[2] 汪晓伟[1] 高明太[3] 李玉民[3] 袁文臻[1] 

机构地区：[1]兰州医学院第一附属医院肿瘤外科,甘肃兰州730000 [2]兰州医学院病原生物学教研室,甘肃兰州730000 [3]兰州医学院第-附属医院普外科,甘肃兰州730000

出　　处：《中国癌症杂志》2003年第4期301-303,共3页China Oncology

基　　金：甘肃省科学事业费项目(编号:QS992-C33-023-14)

摘　　要：目的:探索取材于乳腺癌患者外周血诊断微转移的方法。方法:66例乳腺癌和37例乳腺良性疾病患者的外周血,分离其有核细胞后进行细胞总RNA的抽提,运用RT-PCR技术进行CK-19 mRNA的检测。结果:以RT-PCR终产物出现460bp条带定为阳性。66例乳腺癌患者CK-19mRNA有24例表达,总阳性率为36.36％。37例乳腺良性疾病者CK-19mRNA无一例表达。两组有统计学意义(x2=17.54,P<0.001)。结论:RT-PCR检测CK-19 mRNA可以用于临床诊断乳腺癌患者外周血中微转移。Purpose: Detect cytokeratin 19 mRNA(CK-19 mRNA) in nucleated cells in peripheral blood from breast cancer. To establish a diagnostic method for breast cancer metastasis in peripheral blood. Methods: Peripheral blood samples in breast cancer patients (test group, n = 66) and benign tumour in breast patients ( control group, n = 37) were taken. Then, the nucleated cells were separated and total RNA extracted, and CK-19 mRNA was detected by reverse transcription polymerase chain reaction (RT-PCR) technique. Results: Samples were diagnosed CK-19 mRNA positive when 460 bp band appeared in RT-PCR end-product. The positive rate of CK-19 mRNA is 36. 36 % (24/66) in test group . None of the benign tumour breast patients expressed CK-19 mRNA. The difference between the two groups was statistically significant (P <0.001). Conclusions: RT-PCR can be used to diagnose breast cancer micro metastasis.

关 键 词：应用 RT-PCR检测 乳腺癌 外周血 CK-19MRNA 微转移 

分 类 号：R737.9[医药卫生—肿瘤] R73-37[医药卫生—临床医学]