雌激素对人乳腺癌细胞株MCF7增殖的促进作用  

Influence of estrogen on the proliferation of MCF7, a human breast carcinoma cell line

作　　者：沈敏雄[1] 吴衡[2] 徐贞[2] 梁玉龙[2] 段玲玲[2] 王丽影[2] 查锡良[2] 

机构地区：[1]浙江湖州师范学院医学院基础医学部,浙江湖州313000 [2]复旦大学上海医学院生物化学与分子生物学系,上海200032

出　　处：《中国癌症杂志》2003年第4期308-309,313,共3页China Oncology

摘　　要：目的:观察雌二醇对乳腺癌细胞增殖的促进作用和可能的机制。方法:经不同浓度的雌二醇处理乳腺癌细胞株MCF7后,用MTT比色试验观察雌二醇对MCF7增殖活性的影响,免疫印迹法测量多种与细胞周期、抑癌、增殖、凋亡相关的蛋白质。结果:经MTT比色试验检测,雌二醇能增加MCF7细胞株的增殖活性,细胞周期素-D1明显增加。但雌二醇对抑癌基因PTEN的蛋白表达没有明显影响,以及对具有抗细胞凋亡作用的PKB蛋白量也无明显作用。结论:雌二醇可促进乳腺癌细胞的增殖,可能通过细胞周期素-D1介导的分子机制,而不涉及PKB抗凋亡作用。Purpose: To study the influence of estrogen on the proliferation of human carcinoma cells. Methods: Treated with different concentrations of estrogen, the influence of estrogen on MCF7 was detected by MTT test. Several proteins related with cell cycle, tumor suppressor, proliferation and apoptosis were detected by the method of Western blot. Results: MTT test showed that estrogen could promote the proliferation of MCF7. Cyclin Dl was increased at the same time. But the protein level of PTEN, a tumor suppressor gene, was not changed by estrogen. Furthermore, as an anti -apoptotic protein, PKB protein level was also unchanged. Conclusions: Estrogen can promote the proliferation of human carcinoma cells by certain pathways including cyclin Dl, but not including PKB.

关 键 词：雌激素 乳腺癌 MCF7增殖 促进作用 培养的肿瘤细胞 

分 类 号：R737.9[医药卫生—肿瘤]