多烯紫杉醇诱导SPC-A1肺癌细胞凋亡与活性氧之间的关系  

Relationship between the effect of docetaxel inducing apoptosis of non - small - cell lung cancer cell and the intracellular level of reactive oxygen

作　　者：任振义[1] 白春学[1] 金一尊[2] 洪群英[1] 

机构地区：[1]复旦大学附属中山医院呼吸病研究所,上海200032 [2]复旦大学放射医学研究所,上海200032

出　　处：《中国癌症杂志》2003年第4期342-344,共3页China Oncology

摘　　要：目的:探索多烯紫杉醇诱导SPC-A1肺癌细胞凋亡与细胞内活性氧之间的关系。方法:用含有10-8mol/L多烯紫杉醇作用于体外培养的SPC-A1肺癌细胞24小时后,使用电子显微镜和荧光显微镜检测凋亡细胞形态;以2’,7’-二氯荧光素二乙酸盐和二氢乙啶对上述处理的细胞进行双染后,利用流式细胞仪测定细胞内活性氧水平和细胞周期。结果:10-8mol/L多烯紫杉醇作用于SPC-A1肺癌细胞24小时后,在电子显微镜和荧光显微镜下出现了典型的凋亡细胞形态。流式细胞仪检测结果提示,细胞内活性氧水平较对照组增高,G2～M期细胞在整个细胞群中所占比例增加。结论:多烯紫杉醇通过将肺癌细胞阻滞于G2～M期并且使细胞内产生过多的活性氧诱导细胞凋亡来发挥抗癌作用。Purpose: To explore the relationship between the effect of docetaxel inducing apoptosis of non-small-cell lung cancer cell and intracellular level of reactive oxygen. Methods: SPC-AI lung cancer cells were treated with 10 -8mol/L docetaxel for 24 hours to observe the apoptotic morphological change under the electromicroscope and fluorescence microscope in ritro. The treated cells were harvested to analyze the cell cycles using flow cytometry and to estimate the intracellular reactive oxygen levels by staining with 2', 7-dichlorodihydrofluorescein diacetate and dihydroethidine. Results: After SPC-AI lung cancer cells were exposed to 10 ~ mol/L docetaxel for 24 hours, the typical apoptotic morphological changes were observed under the electromicroscope and fluorescence microscope. There was a higher reactive oxygen level in docetaxe-treated cells group than in control group. After 24 hours docetaxel exposure, there was a significant G2 ~'M phase arrest of SPC-AI lung cancer cells. Conclusions: Docetaxel can induce apoptosis in SPC-AI lung cancer cells through the mechanism of G2 ~ M phase arrest and by elevating intracellular level of reactive oxygen.

关 键 词：多烯紫杉醇 诱导 SPC-A1 肺癌 细胞凋亡 活性氧 

分 类 号：R73-361[医药卫生—肿瘤] R734.2[医药卫生—临床医学]