聚氨酯泡沫固定化重组枯草杆菌三段培养生产青霉素酰化酶  

PRODUCTION OF PENICILLIN G ACYLASE IN THREE-STAGE CONTINUOUS CULTIVATION SYSTEM WITH PUF IMMOBILIZED RECOMBINANT B.subtilis

作　　者：徐志南[1] 陈新爱[2] 岑沛霖[1] 

机构地区：[1]浙江大学生物工程研究所,浙江杭州310027 [2]浙江大学生命科学学院,浙江杭州310029

出　　处：《化工学报》2003年第8期1169-1172,共4页CIESC Journal

摘　　要：Recombinant B subtilis MU108 was immobilized in PUF matrix for enhanced production of PGA The results showed that PUF matrix was suitable for partial immobilization of recombinant cells in a three phase bioreactor With immobilized bacteria, the expression of PGA and the growth of cells were compared between batch cultivation in three phase fluidized bed and continuous bioprocess in three stage cultivation system The productivity of PGA in batch cultivation was improved significantly with the increase of aeration A new three stage continuous cultivation system was designed and tested.It separated the whole process into three stages: cell growth, high temperature induction and PGA expression After the beginning of nutrient continuous feed at mid or late log phase,cell density, pH and residual glucose in the first and third stage bioreactor would be maintained steady up to 65 h Compared with those in the batch fermentation, the average productivity of PGA was improved by 190% in the three stage continuous fermentation, and the time for PGA expression was also extended from ca.10 h to ca 60h These results suggested that this new three stage cultivation technique with immobilized cells might be very efficient for large scale production of heterogeneousRecombinant B subtilis MU108 was immobilized in PUF matrix for enhanced production of PGA The results showed that PUF matrix was suitable for partial immobilization of recombinant cells in a three phase bioreactor With immobilized bacteria, the expression of PGA and the growth of cells were compared between batch cultivation in three phase fluidized bed and continuous bioprocess in three stage cultivation system The productivity of PGA in batch cultivation was improved significantly with the increase of aeration A new three stage continuous cultivation system was designed and tested.It separated the whole process into three stages: cell growth, high temperature induction and PGA expression After the beginning of nutrient continuous feed at mid or late log phase,cell density, pH and residual glucose in the first and third stage bioreactor would be maintained steady up to 65 h Compared with those in the batch fermentation, the average productivity of PGA was improved by 190% in the three stage continuous fermentation, and the time for PGA expression was also extended from ca.10 h to ca 60h These results suggested that this new three stage cultivation technique with immobilized cells might be very efficient for large scale production of heterogeneous proteins

关 键 词：青霉素G酰化酶 重组枯草杆菌 三段培养 固定化 

分 类 号：TQ953[化学工程]