对氧磷酶基因192位点多态性与动脉粥样硬化性脑梗死的关系  

Relationship between the paraoxonase gene 192 polymorphism and atherosclerotic cerebral infarction

作　　者：陈锦华 曾庆杏[2] 文芳[2] 

机构地区：[1]长治市人民医院干部病房,山西省长治市046000 [2]武汉大学人民医院神经内科,湖北省武汉市430060

出　　处：《中国临床康复》2003年第22期3036-3037,共2页Chinese Journal of Clinical Rehabilitation

摘　　要：目的:研究动脉粥样硬化性脑梗死与血清对氧磷酶(PON1)基因192位点多态性的关系。方法:应用聚合酶链式反应-限制性片段长度多态性(PCR-RFLP)方法,对52例动脉粥样硬化性脑梗死患者,48例正常人的PON1基因192位点多态性进行分析,同时检测血脂水平。结果:脑梗死组及正常组均以QR基因型为主,频率分别为0.50,0.54,QQ,RR基因型频率分别为0.10、0.25,0.40、0.21,两组人群基因型分布处于Hardy-Weinberg平衡。脑梗死组R等位基因频率明显高于正常组,分别为0.65,0.48(χ2=4.18P<0.05)。且脑梗死组血清胆固醇、三酰甘油、载脂蛋白B水平高于正常组,低密度脂蛋白胆固醇有升高趋势,而高密度脂蛋白胆固醇、载脂蛋白A1低于正常组。结论:PON1基因192位点多态性可能与动脉粥样硬化性脑梗死有关。R等位基因可能为动脉粥样硬化性脑梗死的相对危险因素。且血脂异常参与脑梗死的发病。AIM:To explore the r elationship between the paraoxonase(PON 1 )gene 192polymorphism and atherosclerotic c erebral infarction(ACI ). METHODS:Using polymerase chain reaction(PCR)-restrict fragment length polym orphism(RLFP),the genotype and allele frequency o f PON 1 gene 192polymorphism were assessed in 52patients with ACI,33males an d19females with an average age of (66±11)years,and 48healthy control s,30males and 18females with the avera ge age of(59±7)years.Serum li pids were measured at the same time .RESULTS:Both in the ACI group and control grou p,the QR genotype of PON 1 gene was the major one,and its frequencies were 0.50and 0.54re-spe ctively.The frequencies of QQ an d RR genotype were 0.10and 0.40respect ively in the ACI group,and 0.25and 0.21respectively in the contr ol gro up.The distribution of genotypes in the two groups was at the Hardy-Weiberg eq uilibriums.But th e frequency of R allele of PON 1 gene in the ACI group was 0.65,significantl y higher than that in the c ontrol grou p(0.48,χ 2 =4.18,P<0.05).Compared with the control group,th e levels of s erum TC,TG and ApoB in the ACI grou p were significantly higher,while the leve ls of serum HDL-C,ApoA1were significantly lower.

关 键 词：对氧磷酶 192位点 基因多态性 动脉粥样硬化性脑梗死 血清 聚合酶链式反应 检测 

分 类 号：R743.33[医药卫生—神经病学与精神病学] R394[医药卫生—临床医学]