肥大细胞在大鼠胰腺纤维化形成中的介导作用  

Mediating effects of mast cells on the progression of pancreatic fibrogenesis in rats with chronic pancreatitis

作　　者：吴恺[1] 张汝玲[1] 王兴鹏[1] 

机构地区：[1]上海交通大学附属第一人民医院消化科,上海200080

出　　处：《胰腺病学》2003年第3期129-132,共4页Chinese JOurnal of Pancreatology

基　　金：上海市科技发展基金 ( No. 0 14 1190 65 )

摘　　要：目的　动态观察大鼠实验性胰腺纤维化过程中胰腺组织肥大细胞的数量和分布变化 ,并探讨其在胰腺纤维化形成中的可能介导作用。方法　经胆胰管注射 2 %三硝基苯磺酸 (TNBS)诱导大鼠胰腺纤维化模型 ,分别在术后 3d、1周和 4周处死大鼠并留取胰腺标本 ,HE染色观察胰腺组织病理学改变和纤维化程度 ;免疫组织化学染色和硫堇蓝染色观察胰腺星状细胞活化及肥大细胞的数量和分布。结果　 HE染色显示 2 % TNBS注射后 3d,胰腺组织病理表现以炎症为主 ,4周时可见明显的胰腺组织纤维化形成。术后 3d组织间质中的肥大细胞数量增加 ,1周后明显增多 (P<0 .0 5 ) ;4周时脱颗粒现象明显增多 ;而在其它区域肥大细胞的数量和分布与 3d时相比未见明显差异 (P>0 .0 5 )。第 4周可见坏死纤维化区域数量较 1周时显著增加 (P<0 .0 1) ,α- SMA在正常组织中呈散在阳性分布 ,术后 3d时呈弱阳性表达 ,1周后阳性明显增强 ,主要位于坏死纤维化区域和胰管周围 ,4周时阳性明显增强 ,多位于纤维化区域。结论　肥大细胞数量增多并活化脱颗粒可能参与了胰腺纤维化的形成过程 ,该作用可能与其脱颗粒释放细胞因子 ,继而刺激胰腺星状细胞活化有关。Objective To investigate the number and distribution of mast cells (MCs) and their potential role in the progression of trinitrobenenze sulfonic acid (TNBS) induced pancreatic fibrosis in rats. Methods The rat pancreatic fibrosis model was established by infusion of 2% TNBS ethyl alcohol solution into the cholangiopancreatic duct. The animals were sacrificed on day 3, week 1 and 4 after operation. The pathologic changes and the extent of fibrosis of the pancreas were studied by H & E staining. MCs in the pancreas were stained by thionine blue, and activation of PSCs, which was indicated by measurement of α SMA, was determined by immunochemistry. Results Inflammation was the major histopathologic change in the pancreas after injection of TNBS solution and interstitial fibrosis was increasingly noted later. On day 3, the number of MCs was increased, mainly localized in the interstitial of the pancreas. At week 1, the number of MCs increased significantly ( P < 0.05 vs 3 d ), and the MCs distributed predominantly in the fibrotic area. At 4 week, the number of MCs increased significantly ( P < 0.01 vs 1 week), and the percentage of degranulation was also increased in the fibrotic area, but in the other area the number and distribution of MCs remained unchanged ( P > 0 05 vs 3 d). The staining of α SMA of PSCs was mildly positive at 3 d, but the positivity became more evident after 1 week, mainly in the fibrous area and the pancreatic duct. The expression of α SMA of PSCs in the normal area was dispersed. Conclusions Activation of MCs might play a mediating regulatory role in the progress of pancreatic fibrosis through degranulation. This action is possibly related to the release of cytokines, which stimulate the activation of PSCs.

关 键 词：肥大细胞 大鼠 胰腺纤维化形成 介导作用 组织病理学 

分 类 号：R576[医药卫生—消化系统]