大蹼铃蟾抗菌肽-2对膀胱癌细胞株HLA-ABC以及DR表达影响的对照实验研究  

The comparative experiment of maximin-2 on expression of HLA-DR HLA-ABC by cultured bladder cancer cell lines

作　　者：申吉泓[1] 官润云[1] 张建华[1] 李文辉[2] 张云[2] 

机构地区：[1]昆明医学院第一附属医院泌尿外科,昆明650032 [2]中国科学院昆明动物研究所"知识创新工程"基地"动物活性蛋白多肽组学"实验室,昆明650032

出　　处：《中国药理学通报》2003年第9期995-998,共4页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助课题 No 3 0 170 195;中国科学院知识创新工程基金

摘　　要：目的　探讨大蹼铃蟾抗菌肽 2 (Maximin 2 )对膀胱癌细胞株HLA DR及HLA ABC表达的影响 ,并与TNF α、IFN α进行比较。方法　采用肿瘤细胞培养方法 ,流式细胞仪检测各实验样品对膀胱癌细胞株T2 4、BIU 87、SCaBER的HLA DR及HLA ABC表达的影响。结果　Maximin 2对不同的膀胱癌细胞株在小剂量下即有抑制作用 ,并呈剂量相关性 ,各实验样品未见对各膀胱癌细胞株的HLA DR表达有影响 ,Maximin 2、TNF α对HLA ABC的表达均无影响 ;IFN α则对HLA ABC的表达有上调作用。结论　Maximin 2、TNF α抗癌机制与提高肿瘤细胞HLA DR、ABC的表达无关 ,IFN α可通过提高肿瘤细胞HLAAIM To study the boosting effect of Maximin 2 on expression of HLA DR and HLA ABC by cultured bladder cancer cell lines and compare with TNF α?IFN α. METHODS Cell culture and FCM were used to dectect the effects of Maximin 2,TNF and IFN on the cell growth of three types of bladder cancer cell lines and HLA DR and HLA ABC expression under the IC 50 culture concentration. RESULTS Maximin 2 had show inhibition to cells growth under the small dosage. TNF α?IFN α also had the effect on restrain all 3 cell lines growth. The expression of HLA DR was not any change in all 3 cell lines after being stimulated by Maximin 2?TNF α and IFN α, Maximin 2 and TNF α can not change HLA ABC expression, IFN α can up regulate HLA ABC expression. CONCLUSION Maximin 2 show the dose dependent effect on cell growth suppression; The mechanism of antitumor of Maximin 2 seems not relate to the enhanced expression of HLA DR or HLA ABC, TNF α and IFN α also not found effect to HLA DR expression. Since IFN α are able to increase the expression of HLA ABC expression,enhanced recognization and cytotoxity of CTL to bladder cancer cells. It is may be one of the mechanism of antitumor of IFN α.

关 键 词：HLA 大蹼铃蟾 抗菌肽 肿瘤坏死因子-α 干扰素-Α 膀胱 

分 类 号：R282.74[医药卫生—中药学] R392.11[医药卫生—中医学]