鼻咽癌活检组织SF_2的检测及其临床意义  

Detection of survival fraction at 2 gray of bioptic tissue from nasopharyngeal carcinoma

作　　者：江浩[1] 夏云飞[1] 钱剑扬[1] 崔念基[1] 梁永钜[2] 

机构地区：[1]中山大学肿瘤防治中心放疗科 [2]中山大学肿瘤药物基础研究室,广东广州510060

出　　处：《蚌埠医学院学报》2003年第5期393-396,共4页Journal of Bengbu Medical College

基　　金：中山大学 (原中山医科大学 ) 2 11工程资助项目 (编号 :980 5 9)

摘　　要：目的 :用MTT法显色图像分析技术测定鼻咽癌活检组织 2Gy照射后的细胞存活分数 (survivalfractionat 2 grey ,SF2 )值并初步探讨其意义。方法 :取中山大学肿瘤医院门诊鼻咽癌患者新鲜的鼻咽部活检病理标本 4 0例 ,用组织块原代培养MTT法显色图像分析技术测定活检肿瘤组织的SF2 。结果 :4 0例中 36例病理皆为非角化癌 ,36例患者测定的SF2 分布为0 .12～ 0 .99,中位数 0 .5 7,且这种差异和患者的年龄、性别、肿瘤的T分期、肿瘤的N分期无明显关联 (P >0 .0 5 )。结论 :用该技术测定鼻咽癌患者肿瘤组织的SF2 值 ,可以反映出相同病理类型的不同患者病理组织SF2 的差别 ,其方法简便快捷、有效可行。Objective:To explore the method of detecting survival fraction at two gray(SF 2) of nasopharyngeal carcinoma and its significance.Methods:SF 2 of the fresh bioptic specimen from 40 patients with nasopharyngeal carcinoma in the out patient department of the Affiliated Tumor Hospital of Sun Yat sen University was estimated by means of image analysis of the tissue fragment primary culture.The pathologic type of 36 cases was non keratinizing carcinoma.Results:The value of SF 2 ranged from 0.12 to 0.99,and the median was 0.57.This value was probably independent of the age,gender,T stage of and N stage of tumor( P >0.05).Conclusions:The SF 2 detected by this technique may show the difference among patients with the same pathologic type of nasopharyngeal carcinoma,and the method is convenient and reasonable.

关 键 词：鼻咽肿瘤 放射敏感性 原代培养 细胞存活分数 图像分析技术 

分 类 号：R739.63[医药卫生—肿瘤]