Effect of Intravascular Irradiation on Intimal Smooth Muscle Cells Proliferation and Apoptosis in Balloon Injuried Arteries  

血管内放射对猪受损髂动脉平滑肌细胞增殖与凋亡的影响(英文)

作　　者：马根山[1] 王连生[1] 黄峻[1] 冷静[2] 彭韬[2] 杨志健[1] 尹航[1] 曹克将[1] 

机构地区：[1]南京医科大学第一附属医院心脏科,南京210029 [2]南京医科大学病理系

出　　处：《Journal of Nanjing Medical University》2003年第4期159-165,共7页南京医科大学学报（英文版）

基　　金：GrantfromNaturalScienceFoundationofJiangsuProvince (DA990 1)

摘　　要：Objective: To investigate the effect of intravascular in radiation on thearterial wall smooth muscle cells (SMCs) proliferation and apoptosis after iliac artery bollominjury in figs. Methods: Twenty-seven miniature figs were divided into three groups. All pigsunderwent iliac artery balloon over-stretch. An^(192) Ir source through afterloader was positionedat the injuried segments to give 10 Gy in 9 pigs and 20 Gy in the other 9 pigs, and the rest 9 pigswere, used as control group. The pigs were killed on the 3rd, 10th and 28th days respectively forobservation. The injured segments were processed to examine SMCs proliferation by proliferation cellnuclear antigen (PCNA) and apopto-sis by terminal deoxynucleotidyl transferase-mediated dUTPnick-end labeling (TUNEL). Results: PC-NA index analysis has some that SMCs proliferation inneointima was significantly inhibited in irradiation group on the 10th and 28th days. The value forintimal SMCs apoptosis in control vs 10 Gy and 20 Gy irradiation groups were: (1. 185+-0. 49)% vs(2. 27+-0. 49)%(P>0. 05) and (1. 85+-0. 49)% vs (2. 53+-0. 45)%(P<0. 05), at the 10th day; (1.61+-0. 35)% vs (3. 11+-0. 51)%(P<0. 05), and (1.61+-0. 35)% vs (7. 05+-1. 82)% (P<0. 05), on the28th day. In irradiated arteries, the maximal incidence of intimal SMCs apoptosis was (7. 05+--1.82)% in 20 Gy group vs (3. 11+-0. 51)% in 10 Gy group (P<0. 05), on the 28th day. In the same doseirradiation group, the incidence of intimal SMCs apoptosis was higher on the 28th day than that onthe 10th day. Conclusion: Intra-arterial gamma irradiation can inhibit intimal SMCs proliferationand stimulate SMCs apoptosis in balloon-in jured arteries. These may be contributive to preventionof restenosis of arteries after balloon injury.目的 :观察血管内放射照射对猪髂动脉球囊损伤后血管新生内膜平滑肌细胞(SMC)增殖与凋亡的影响。方法 :2 7头小型猪分为 3组 ,所有猪行髂动脉球囊过大扩张 ,通过后装装置将 1 0Gy和 2 0Gy的192 Ir放射源分别置于 9只猪受损髂动脉部位 ,其他 9只猪的受损髂动脉作为对照。每组的 9头猪分别术后 3天、1 0天和 2 8天分 3次处死。用增殖细胞核抗原(PCNA)和三磷酸脱氧尿嘧啶缺口末端标记法 (TUNEL)法检测内膜SMC增殖和凋亡情况。结果 :代表血管内膜增殖的PCNA指数在 1 0天和 2 8天宰杀的猪中 ,放射治疗组明显低于对照组。术后 1 0天内膜SMC凋亡在对照组和 1 0Gy、2 0Gy组的值分别为 :(1 85± 0 49) %比 (2 2 7± 0 49) %(P >0 0 5)和 (1 85± 0 49) %比 (2 53± 0 45) %(P <0 0 5) ;术后 2 8天上述值分别为(1 61± 0 3 5) %比 (3 1 1± 0 51 ) %(P <0 0 5)和 (1 61± 0 3 5) %比 (7 0 5± 1 82 ) %(P <0 0 5) ;2 8天时 2 0Gy组的内膜SMC凋亡明显高于 1 0Gy组 (7 0 5± 1 82 ) %比 (3 1 1± 0 51 ) %(P <0 0 5)。相同的放射剂量时 ,2 8天宰杀猪的髂动脉SMC凋亡高于 1 0天宰杀猪的量。结论

关 键 词：intravascular irradiation smooth muscle cells PROLIFERATION APOPTOSIS RESTENOSIS 

分 类 号：R543.5[医药卫生—心血管疾病]