肝癌细胞裂解物致敏DC疫苗诱导的肿瘤特异抗瘤效应  

The tumor specific anti-tumor effects mounted by hepatoma cell lysates pulsed DC

作　　者：邱双健[1] 叶胜龙[1] 汤钊猷[1] Shiguang Qian Lina Lu 

机构地区：[1]200032,上海,复旦大学肝癌研究所中山医院外科 [2]美国匹兹堡大学器官移植研究所

出　　处：《中华实验外科杂志》2003年第9期797-798,共2页Chinese Journal of Experimental Surgery

基　　金：国家重点基础研究 (973)项目 (G1 9980 51 2 0 0 );国家"九五"科技攻关项目 (96 90 6 0 1 2 0 );国家自然科学基金资助项目(39770 82 6)

摘　　要：目的　研究肝癌细胞裂解物致敏的树突状细胞 (DC)疫苗诱导的体内、外抗瘤效应。方法　体外实验 :将肿瘤细胞裂解物致敏的DC与T细胞共培养 ,4d后收获致敏的T细胞 ,观察其对野生型肿瘤细胞的杀伤反应并检测培养液中的IFN γ分泌情况。体内实验 :观察肿瘤细胞裂解物致敏的DC疫苗对小鼠皮下肝癌发生的抑制作用。结果　在效 :靶比分别为 5 0 .0∶1.0、2 5 .0∶1.0、12 .5∶1.0时 ,肝癌细胞裂解物致敏的DC组与DC组诱导的肿瘤杀伤活性分别为 (4 5 .7±3 .2 ) %对 (2 6.5± 2 .5 ) % ;(3 1.0± 2 .7) %对 (14 .3± 3 .3 ) % ;(2 7.8± 1.7) %对 (9.9± 0 .6) % (在各效靶比 ,P <0 .0 1) ,混合细胞培养上清液中IFN γ浓度则为 (2 60 3 .3± 2 60 .0 )ng/L对 (5 0 1.0±5 0 .0 )ng/L。在体内则可有效抑制小鼠皮下肝癌的发生。Objective To study the anti-tumor effects elicited by hepatoma cell lysates pulsed DC vaccine both in vitro and in vivo.Methods For in vitro study,the sensitized T cells were harvested 4 days after co-culture of tumor cell lysates pulsed DC with naive T cells and used as effectors to observe the cytotoxicity against the wild type tumor cells.The expression levels of IFN-γ in the supernatant of co-culture were detected by ELISA method.For in vivo study,the preventive effects of tumor cell lysates pulsed DC vaccine were evaluated in a murine hepatocellular carcinoma model.Results The tumor lysis ratios (%) in pulsed DC vaccine group were higher than in DC vaccine group (45.7±3.2 vs 26.5±2.5, 31.0±2.7 vs 14.3±3.3,27.8±1.7 vs 9.9±0.6 at ratios of 50.0∶1.0,25.0∶1.0,12.5∶1.0 effector/target),respectively.The IFN-γ (ng/L) expression level in the supernatant in pulsed DC vaccine group (2?603.3±260.0) was also higher than that in DC vaccine group (501.0±50.0).The in vivo growth of tumor could be inhibited effectively by pulsed DC vaccine.Conclusion The hepatoma cell lysates pulsed DC vaccine can induce tumor specific anti-tumor effects.

关 键 词：肝癌 细胞裂解物致敏 树突状细胞 疫苗诱导 肿瘤特异抗瘤效应 

分 类 号：R735.7[医药卫生—肿瘤] R730.3[医药卫生—临床医学]